Home / ADAM10 / Human ADAM10 Ectodomain Antibody

Human ADAM10 Ectodomain Antibody

Catalog #: MAB1427
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1427-SP
MAB1427-100
MAB1427-500
Product Details
Citations (19)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM10 Ectodomain Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAM10 in direct ELISAs and Western blots. In Western blots, shows less than 5% cross-reactivity with recombinant human (rh) ADAM8, 9, 15, 17/TACE, and recombinant mouse ADAM10.
Source
Monoclonal Mouse IgG2B Clone # 163003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ADAM10
Thr214-Glu672
Accession # O14672
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ADAM10 (Catalog # 936-AD)
under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAM10 (Catalog # 936‑AD), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM10

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

References
  1. Rooke, et al. (1996) Science 273:1227.
  2. Pan and Rubin (1997) Cell 90:271.
  3. Qi, et al. (1999) Science 283:91.
  4. Lammich, et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
  5. Rosendahl, et al. (1997) J. Biol. Chem. 272:24588.
  6. Chantry, et al. (1989) J. Biol. Chem. 264:21603.
  7. Millichip, et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
  8. Chantry and Glynn (1990) Biochem. J. 268:245.
  9. Fahrenholz, et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Entrez Gene IDs
102 (Human); 11487 (Mouse)
Alternate Names
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease

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Citations for Human ADAM10 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth
    J Exp Med, 2016-08-08;0(0):.
  2. Fully human monoclonal antibody targeting activated ADAM10 on colorectal cancer cells
    Authors: Nayanendu Saha, Du-San Baek, Rachelle P. Mendoza, Dorothea Robev, Yan Xu, Yehuda Goldgur et al.
    Biomedicine & Pharmacotherapy
  3. ADAM10 mediates shedding of carbonic anhydrase IX ectodomain non?redundantly to ADAM17
    Authors: M Zatovicova, I Kajanova, M Takacova, L Jelenska, O Sedlakova, M Labudova, S Pastorekov
    Oncology reports, 2022-12-16;49(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Quantitative Proteomics Identifies Proteins Enriched in Large and Small Extracellular Vesicles
    Authors: Anna Lischnig, Markus Bergqvist, Takahiro Ochiya, Cecilia Lässer
    Molecular & Cellular Proteomics
  5. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV‐1 infection
    Authors: Lorena Martin‐Jaular, Nathalie Nevo, Julia P Schessner, Mercedes Tkach, Mabel Jouve, Florent Dingli et al.
    The EMBO Journal
  6. Microbiota-derived short chain fatty acids modulate microglia and promote A beta plaque deposition
    Authors: Alessio Vittorio Colombo, Rebecca Katie Sadler, Gemma Llovera, Vikramjeet Singh, Stefan Roth, Steffanie Heindl et al.
    eLife
  7. Isolation and characterization of extracellular vesicle subpopulations from tissues
    Authors: R Crescitell, C Lässer, J Lötvall
    Nature Protocols, 2021-01-25;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Extracellular vesicles containing ACE2 efficiently prevent infection by SARS‐CoV‐2 Spike protein‐containing virus
    Authors: Federico Cocozza, Nathalie Névo, Ester Piovesana, Xavier Lahaye, Julian Buchrieser, Olivier Schwartz et al.
    Journal of Extracellular Vesicles
  9. S. aureus alpha-toxin monomer binding and heptamer formation in host cell membranes - Do they determine sensitivity of airway epithelial cells toward the toxin?
    Authors: N Möller, S Ziesemer, P Hildebrand, N Assenheime, U Völker, JP Hildebrand
    PLoS ONE, 2020-05-29;15(5):e0233854.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Non-cell-autonomous function of DR6 in Schwann cell proliferation
    Authors: A Colombo, HE Hsia, M Wang, PH Kuhn, MS Brill, P Canevazzi, R Feederle, C Taveggia, T Misgeld, SF Lichtentha
    EMBO J., 2018-02-19;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Ectodomain Shedding of the Cell Adhesion Molecule Nectin-4 in Ovarian Cancer is Mediated by ADAM10 and ADAM17
    Authors: PC Buchanan, KL Boylan, B Walcheck, R Heinze, MA Geller, PA Argenta, AP Skubitz
    J. Biol. Chem, 2017-02-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration
    Authors: Zhenyu Cai, Anling Zhang, Swati Choksi, Weihua Li, Tao Li, Xue-Min Zhang et al.
    Cell Research
  13. ADAM10 new selective inhibitors reduce NKG2D ligand release sensitizing Hodgkin lymphoma cells to NKG2D-mediated killing
    Authors: Maria Raffaella Zocchi, Caterina Camodeca, Elisa Nuti, Armando Rossello, Roberta Venè, Francesca Tosetti et al.
    OncoImmunology
  14. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
    Authors: Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
    J Immunol, 2015-06-12;195(2):736-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1).
    Authors: Breshears L, Schlievert P, Peterson M
    J Biol Chem, 2012-07-25;287(39):32578-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas
    Authors: Maria Raffaella Zocchi, Silvia Catellani, Paolo Canevali, Sara Tavella, Anna Garuti, Barbara Villaggio et al.
    Blood
  17. Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases.
    Authors: Koenen RR, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, Reiss K, Sarabi A, Lindbom L, Hackeng TM, Weber C, Ludwig A
    Blood, 2009-03-03;113(19):4799-809.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans.
    Authors: Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB
    Cell, 2005-10-21;123(2):291-304.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Western Blot
  19. Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes.
    Authors: Johansson MW, Lye MH, Barthel SR, Duffy AK, Annis DS, Mosher DF
    Am. J. Respir. Cell Mol. Biol., 2004-06-25;31(4):413-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC

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