Human ADAM15 Ectodomain Antibody Summary
Asp207-Thr696
Accession # Q13444
Applications
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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ADAM15 in MCF‑7 Human Cell Line. ADAM15 was detected in immersion fixed MCF-7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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ADAM15 in Human Prostate Cancer Tissue. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of ADAM15 in PC‑3 cells (Positive) & MOLT‑4 cells (Negative). ADAM15 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in MOLT‑4 human acute lymphoblastic leukemia cells (Negative) using Mouse Anti-Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human ADAM15 by Flow Cytometry miR-147b down-regulates cell surface expression of ADAM15 in endothelial cells.At 48 h after transfection, live HUVECs were labeled with a monoclonal anti-ADAM15 ectodomain antibody and a secondary Cy3 conjugate. Cell surface expression was analyzed by flow cytometry (A–B). The bar graph shows quantitative cell surface ADAM15 expression in different treatment groups, each derived from 3 separate experiments (C). Data represent mean ± SEM. * P<0.05 vs. untreated control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25333931), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human ADAM15 by Flow Cytometry miR-147b down-regulates cell surface expression of ADAM15 in endothelial cells.At 48 h after transfection, live HUVECs were labeled with a monoclonal anti-ADAM15 ectodomain antibody and a secondary Cy3 conjugate. Cell surface expression was analyzed by flow cytometry (A–B). The bar graph shows quantitative cell surface ADAM15 expression in different treatment groups, each derived from 3 separate experiments (C). Data represent mean ± SEM. * P<0.05 vs. untreated control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25333931), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM15
ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.
Product Datasheets
Citations for Human ADAM15 Ectodomain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Chemical fixation creates nanoscale clusters on the cell surface by aggregating membrane proteins
Authors: Takehiko Ichikawa, Dong Wang, Keisuke Miyazawa, Kazuki Miyata, Masanobu Oshima, Takeshi Fukuma
Communications Biology
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MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression.
Authors: Chatterjee V, Beard R, Reynolds J, Haines R, Guo M, Rubin M, Guido J, Wu M, Yuan S
PLoS ONE, 2014-10-15;9(10):e110286.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma.
Authors: Ungerer C, Doberstein K, Burger C, Hardt K, Boehncke WH, Bohm B, Pfeilschifter J, Dummer R, Mihic-Probst D, Gutwein P
Biochem. Biophys. Res. Commun., 2010-09-17;401(3):363-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Homeostatic effects of the metalloproteinase disintegrin ADAM15 in degenerative cartilage remodeling.
Authors: Bohm BB, Aigner T, Roy B, Brodie TA, Blobel CP, Burkhardt H
Arthritis Rheum., 2005-04-01;52(4):1100-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer
Authors: LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang
Cell Rep, 2020-10-13;33(2):108253.
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