Human ADAM15 Ectodomain Antibody Summary
Leu18-Ser693
Accession # Q13444
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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ADAM15 in Human Prostate. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ADAM15 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF935) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM15
ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.
Product Datasheets
Citations for Human ADAM15 Ectodomain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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A Novel Pro-Inflammatory Mechanosensing Pathway Orchestrated by the Disintegrin Metalloproteinase ADAM15 in Synovial Fibroblasts
Authors: T Janczi, F Meier, Y Fehrl, RW Kinne, B Böhm, H Burkhardt
Cells, 2021-10-09;10(10):.
Species: Human
Sample Types: Whole Cells
Applications: IF, Immunoprecipitation -
ADAM-12 as a diagnostic marker for the proliferation, migration and invasion in patients with small cell lung cancer.
Authors: Shao, Shuhong, Li, Zunling, Gao, Wei, Yu, Guohua, Liu, Dexiang, Pan, Fang
PLoS ONE, 2014-01-21;9(1):e85936.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins.
Authors: Kleino I, Ortiz RM, Yritys M, Huovila AP, Saksela K
J. Cell. Biochem., 2009-11-01;108(4):877-85.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ADAM15 upregulation and interaction with multiple binding partners in inflammatory bowel disease.
Authors: Mosnier JF, Jarry A, Bou-Hanna C, Denis MG, Merlin D, Laboisse CL
Lab. Invest., 2006-08-07;86(10):1064-73.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot
FAQs
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Thank you so much for taking the time to share your experience using RnD Systems Antibodies. We value your opinion and use the online reviews as testimonials from our customers, of the quality of our products. Our technical service representatives work hard to resolve any issues with our products when customers report they have not had success. In our QC process, ADAM15 staining was detected in paraffin-embedded sections of human prostate using # AF935 at 5 µg/mL overnight at 4 °C with Anti-Goat HRP-DAB Cell Tissue Staining Kit Catalog # CTS008, using Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. Customer have tested AF935 and MAB935 in their staining. MAB935 works in human lung section and customer doesn’t need further troubleshooting.
