Human ADAM23 Antibody Summary
Ser60-His585
Accession # O75077
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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ADAM23 in Human Spleen. ADAM23 was detected in immersion fixed paraffin-embedded sections of human spleen using 15 µg/mL Mouse Anti-Human ADAM23 Monoclonal Antibody (Catalog # MAB4974) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM23
ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100 kDa member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum, and serves as a counter-receptor for alpha v beta 3 integrin. The human ADAM23 proprecursor is a 773 amino acid (aa) type I transmembrane (TM) protein. It contains a 227 aa cleavable proregion (aa 60‑286) and a 506 aa extracellular domain (ECD) (aa 287‑792) that is part of a 70 kDa mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299‑494), an integrin-binding disintegrin region (aa 511‑585), and a Cys‑rich domain (aa 589‑611). Two splice variants exist. One shows an in-frame 46 aa substitution for aa 787‑832 that generates a soluble form, while a second shows an in‑frame 31 aa TM substitution for aa 787‑817. Over aa 60‑585, human ADAM23 shares 92% aa sequence identity with mouse ADAM23.
Product Datasheets
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