Human ADAM28 Antibody

Catalog #: MAB93252 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB93252-100
MAB93252-SP

Detection of ADAM28 in Human PBMC by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM28 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM28 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1004266

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human ADAM28
Leu72 & Leu191-Ala623,
Accession # Q0UKQ2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ADAM28 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Mouse Anti-Human ADAM28 Monoclonal Antibody (Catalog # MAB93252) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM28

A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3). ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4 beta 1(9). R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.

References

Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
Howard, L. et al. (2000) Biochem. J. 348:21.
Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
Blobel, C.P. (1997) Cell 90:589.
Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.

Long Name

A Disintegrin and Metalloproteinase Domain 28

Entrez Gene IDs

10863 (Human); 13522 (Mouse); 290344 (Rat)

Alternate Names

a disintegrin and metalloproteinase domain 28; ADAM metallopeptidase domain 28; ADAM23MDC-L; ADAM28; disintegrin and metalloproteinase domain-containing protein 28; Dtgn1; EC 3.4.24; EC 3.4.24.-; eMDC II; eMDCII; Epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich proteinII; MDCL; MDCLADAM 28; MDC-Lm; MDC-Ls; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L; Protein-L