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Human ADAM9 Ectodomain Antibody

Catalog #: AF939
7 Citations Datasheet / COA / SDS
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AF939
AF939-SP
Detection of Human ADAM9 by Western Blot.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM9 Ectodomain Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAM9 Ectodomain in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant mouse (rm) rmADAM9 is observed and less than 0.05% cross-reactivity with recombinant human (rh) rhADAM8, rhADAM10, rhTACE, rhTIMP-1, rhTIMP-2, rhTIMP-3, rhTIMP-4, rhBACE-1 and rmADAM10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ADAM9
Ala206-Asp697
Accession # Q13443
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAM9 (Catalog # 939‑AD), see our available Western blot detection antibodies
Knockout Validated
ADAM9 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in ADAM9 knockout HeLa cell line.
 

Human ADAM9 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human ADAM9 Ectodomain Biotinylated Antibody (Catalog # BAF939)

Standard: Recombinant Human ADAM9 Protein, CF (Catalog # 939-AD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ADAM9 antibody by Western Blot. View Larger

Detection of Human ADAM9 by Western Blot. Western blot shows lysates of WI-38 human lung fibroblast cell line and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 110 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated Western Blot Shows Human ADAM9 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human ADAM9 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ADAM9 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ADAM9 at approximately 78 and 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM9

ADAM9, also known as MDC9 or meltrin gamma, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75 TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane-anchored heparin-binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin beta -chain and fibronectin (2,4). Besides its catalytic activity, ADAM9 functions as an adhesion molecule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3 (SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

References
  1. Moss, et al. (2001) DDT 6:417.
  2. Roghan, et al. (1999) J. Biol. Chem. 274:3531.
  3. Izumi, et al. (1998) EMBO J. 17:7260.
  4. Schwettmann and Tschesche (2001) Prot. Expre. & Purif. 21:65.
  5. Nath, et al. (2000) J. Cell Sci. 113:2319.
  6. Zhou, et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
  7. Howard, et al. (1999) J. Biol. Chem. 274:31693.
  8. Weskamp, et al. (1996) J. Cell. Biol. 132:717.
Long Name
A Disintegrin and Metalloprotease-like Domain 9
Entrez Gene IDs
8754 (Human); 11502 (Mouse)
Alternate Names
a disintegrin and metalloproteinase domain 9 (meltrin gamma); ADAM 9; ADAM metallopeptidase domain 9 (meltrin gamma); ADAM metallopeptidase domain 9; ADAM9; Cellular disintegrin-related protein; cone rod dystrophy 9; CORD9; disintegrin and metalloproteinase domain-containing protein 9; EC 3.4.24; EC 3.4.24.-; MCMP; MCMPMDC9KIAA0021Mltng; MDC9; Meltrin gamma; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; MLTNG; Myeloma cell metalloproteinase

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Citations for Human ADAM9 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. The ADAM9/UBN2/AKR1C3 axis promotes resistance to androgen-deprivation in prostate cancer
    Authors: TT Le, CL Hsieh, IH Lin, CY Chu, AD Do, SH Chen, K Shigemura, K Kitagawa, M Fujisawa, MC Liu, KC Chen, SY Sung
    American journal of cancer research, 2022-01-15;12(1):176-197.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Identification of the Cell-Surface Protease ADAM9 as an Entry Factor for Encephalomyocarditis Virus
    Authors: Jim Baggen, Hendrik Jan Thibaut, Daniel L. Hurdiss, Maryam Wahedi, Caleb D. Marceau, Arno L. W. van Vliet et al.
    mBio
  3. ADAM9 is over-expressed in human ovarian clear cell carcinomas and suppresses cisplatin-induced cell death
    Authors: M Ueno, T Shiomi, S Mochizuki, M Chijiiwa, M Shimoda, Y Kanai, F Kataoka, A Hirasawa, N Susumu, D Aoki, Y Okada
    Cancer Sci., 2018-02-01;109(2):471-482.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration
    Authors: Zhenyu Cai, Anling Zhang, Swati Choksi, Weihua Li, Tao Li, Xue-Min Zhang et al.
    Cell Research
  5. Expression profiles and clinical correlations of degradome components in the tumor microenvironment of head and neck squamous cell carcinoma.
    Authors: Stokes A, Joutsa J, Ala-Aho R, Pitchers M, Pennington CJ, Martin C, Premachandra DJ, Okada Y, Peltonen J, Grenman R, James HA, Edwards DR, Kahari VM
    Clin. Cancer Res., 2010-03-21;16(0):2022.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Role of ADAM-9 disintegrin-cysteine-rich domains in human keratinocyte migration.
    Authors: Zigrino P, Steiger J, Fox JW, Loffek S, Schild A, Nischt R, Mauch C
    J. Biol. Chem., 2007-08-17;282(42):30785-93.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr, Western Blot
  7. Transmembrane collagen XVII, an epithelial adhesion protein, is shed from the cell surface by ADAMs.
    Authors: Franzke CW, Tasanen K, Schacke H, Tryggvason K, Mauch C, Zigrino P, Sunnarborg S, Fahrenholz F, Bruckner-Tuderman L
    EMBO J., 2002-10-01;21(19):5026-35.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr

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