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Human ADAMTS5 Antibody

Catalog #: MAB2198
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2198
MAB2198-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAMTS5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAMTS5 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ADAMTS1 or rhADAMTSL1.2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 362810
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ADAMTS5
Ser262-Pro622
Accession # Q9UNA0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ADAMTS5 (Catalog # 2198-AD) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAMTS5 (Catalog # 2198‑AD), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS5

ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as Aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving alpha 2-Macroglobulin (3), Aggrecan (4), and Brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6). Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and rheumatoid arthritis. The purified recombinant human (rh) ADAMTS5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and rhTIMP-3 (R&D Systems, Catalog # 973-TM).

References
  1. Porter, S. et al. (2005) Biochem. J. 386:15.
  2. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  3. Tortorella, M.D. et al. (2004) J. Biol. Chem. 279:17554.
  4. Vankemmelbeke, M.N. et al. (2001) Eur. J. Biochem. 268:1259.
  5. Nakada, M. et al. (2005) Acta Neurophathol (Berl). 110:239.
  6. Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
  7. Glasson, S.S. et al. (2005) Nature, 434: 644.
  8. Stanton, H. et al. (2005) Nature, 434:648.
Long Name
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 5
Entrez Gene IDs
11096 (Human); 23794 (Mouse); 304135 (Rat)
Alternate Names
A disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2); ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADAM-TS 11; ADAM-TS 5; ADAMTS11; ADAMTS-11; ADAMTS5; ADAM-TS5; ADAMTS-5; ADMP2; ADMP-2; ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5; Aggrecanase 2; aggrecanase-2; disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.14; EC 3.4.24.82

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Citation for Human ADAMTS5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Desumoylation of aggrecan and collagen II facilitates degradation via aggrecanases in IL-1 beta -mediated osteoarthritis
    Authors: Bing Zhu, Guanxing Cui, Qifu Zhang, Xiankui Cheng, Shusen Tang
    Journal of Pain Research

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