Human ADAMTSL-1/Punctin Antibody

Catalog # Availability Size / Price Qty
MAB1717
MAB1717-SP
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Human ADAMTSL-1/Punctin Antibody Summary

Species Reactivity
Human
Specificity
Detects human ADAMTSL‑1/Punctin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 212125
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ADAMTSL‑1/Punctin
Glu29-Ser525
Accession # Q8N6G6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ADAMTSL‑1/Punctin
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ADAMTSL‑1/Punctin, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTSL-1/Punctin

ADAMTSL-1, also known as Punctin, is a secreted glycoprotein resembling members of the ADAMTS family although it lacks the propeptide region and the metalloproteinase and disintegrin-like domains. ADAMTSL-1 is expressed in adult skeletal muscle and may have important functions in the extracellular matrix. Alternative splicing of the ADAMTSL-1 transcript results in three isoforms. Isoform1 is the longest and contains the entire isoform 2. Isoform 3 has the same sequence as isoforms 1 and 2 until residue 361, but a unique C-terminal end of 78 amino acids.

Long Name
ADAMTS-like Protein 1
Entrez Gene IDs
92949 (Human)
Alternate Names
ADAM-TS related protein 1; ADAMTSL1; ADAMTSL-1; ADAMTS-like 1; ADAMTS-like protein 1; ADAMTSR1MGC118805; C9orf94; chromosome 9 open reading frame 94; DKFZp686L03130; FLJ35283; FLJ41032; FLJ46891; MGC118803; MGC40193; PUNCTIN; Punctin-1

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