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Human Adenosine A2b R Antibody

Catalog #: MAB10444 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10444-100
MAB10444-SP
Detection of Human Adenosine A2b R by Western Blot.
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Human Adenosine A2b R Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adenosine A2b R in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1022311
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human Adenosine A2b R
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Adenosine A2b R antibody by Western Blot. View Larger

Detection of Human Adenosine A2b R by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and human cerebeullum tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Adenosine A2b R Monoclonal Antibody (Catalog # MAB10444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adenosine A2b R at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2b R

ADORA2B (Adenosine B2a Receptor, Adora-B2) is a widely expressed seven transmembrane G protein-coupled receptor (GPCR) activated by adenosine. Studies show that adenosine is crucial for ischemic preconditioning (IP) via its receptors ADORA1, ADORA2a and ADORA2b. All of these receptors are expressed in the myocardium and have been implicated in the role of cardioprotection during ischemia. Human and mouse Adora2B sequences are 88% identical over the full protein, but only 77% identical on the extra-cellular domains, including N-term tail and three loops.

Long Name
Adenosine A2b Receptor
Entrez Gene IDs
136 (Human); 11541 (Mouse); 29316 (Rat)
Alternate Names
Adenosine A2b R; adenosine A2b receptor; Adenosine A2bR; adenosine receptor A2b; ADORA2; ADORA2B

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