Human Adiponectin/Acrp30 Antibody

Catalog #: MAB10654 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10654-100
MAB10654-SP

Human Adiponectin/Acrp30 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adiponectin/Acrp30 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adiponectin/Acrp30 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 553530R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30
Glu19-Asn244
Accession # Q15848

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Adiponectin/Acrp30 DuoSet ELISA Kit (Catalog # DY1065) for convenient development of a sandwich ELISA or the Human Total Adiponectin/Acrp30 Quantikine ELISA Kit (Catalog # DRP300) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Adiponectin/Acrp30 ELISA Standard Curve. Recombinant Human Adiponectin/Acrp30 protein was serially diluted 2-fold and captured by Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10653) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in non-metabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1‑3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin 13/T-Cadherin, as well as with several growth factors (4‑7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8, 9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10‑12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

References

Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
Yamauchi, T. et al. (2007) Nat. Med. 13:332.
Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
Waki, H. et al. (2005) Endocrinology 146:790.
Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
Wang, Y. et al. (2008) Biochem. J. 409:623.
Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Alternate Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein