Human Adiponectin/Acrp30 Biotinylated Antibody

Catalog #: BAM1065
8 Citations Datasheet / COA / SDS

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BAM1065

Product Details

Citations (8)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adiponectin/Acrp30 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adiponectin in ELISAs. In ELISAs, this antibody shows no cross-reactivity with recombinant mouse Adiponectin. This antibody detects an epitope in the globular domain (amino acids 104 - 244).

Source

Monoclonal Mouse IgG₁ Clone # 166128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Adiponectin
Glu19-Asn244
Accession # Q15848

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human Adiponectin/Acrp30 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human Adiponectin/Acrp30 Antibody (Catalog # MAB10651R)

Capture Reagent: Human Adiponectin/Acrp30 Antibody (Catalog # MAB10651)

Standard: Recombinant Human Adiponectin/Acrp30 Protein, CF (Catalog # 1065-AP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as adipocyte complement-related protein of 30 kDa (Acrp30), is secreted from adipocytes and has been implicated in energy homeostasis and obesity.

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Alternate Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Adiponectin/Acrp30 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Development and validation of cardiometabolic risk predictive models based on LDL oxidation and candidate geromarkers from the MARK-AGE data
Authors: Valeanu, A;Margina, D;Weber, D;Stuetz, W;Moreno-Villanueva, M;Dollé, MET;Jansen, EH;Gonos, ES;Bernhardt, J;Grubeck-Loebenstein, B;Weinberger, B;Fiegl, S;Sikora, E;Mosieniak, G;Toussaint, O;Debacq-Chainiaux, F;Capri, M;Garagnani, P;Pirazzini, C;Bacalini, MG;Hervonen, A;Slagboom, PE;Talbot, D;Breusing, N;Frank, J;Bürkle, A;Franceschi, C;Grune, T;Gradinaru, D;
Mechanisms of ageing and development
Species: Human
Sample Types: Serum
Applications: Functional Assay
The Impact of an 8-Week Supplementation with Fermented and Non-Fermented Aronia Berry Pulp on Cardiovascular Risk Factors in Individuals with Type 2 Diabetes
Authors: Christiansen, CB;Jeppesen, PB;Hermansen, K;Gregersen, S;
Nutrients
Species: Human
Sample Types: Serum
Applications: ELISA Detection
Nutritional Factors Modulating Alu Methylation in an Italian Sample from The Mark-Age Study Including Offspring of Healthy Nonagenarians
Authors: R Giacconi, M Malavolta, A Bürkle, M Moreno-Vil, C Franceschi, M Capri, PE Slagboom, EHJM Jansen, MET Dollé, T Grune, D Weber, A Hervonen, W Stuetz, N Breusing, F Ciccarone, M Zampieri, V Aversano, P Caiafa, L Formentini, F Piacenza, E Pierpaoli, A Basso, M Provincial, M Cardelli
Nutrients, 2019-12-06;11(12):.
Species: Human
Sample Types: Serum
Applications: ELISA Detection
Dopamine Receptors in Human Adipocytes: Expression and Functions
Authors: Dana C. Borcherding, Eric R. Hugo, Gila Idelman, Anuradha De Silva, Nathan W. Richtand, Jean Loftus et al.
PLoS ONE
A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010-06-08;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development
Adiponectin levels measured in dried blood spot samples from neonates born small and appropriate for gestational age.
Authors: Klamer A, Skogstrand K, Hougaard DM, Norgaard-Petersen B, Juul A, Greisen G
Eur. J. Endocrinol., 2007-08-01;157(2):189-94.
Species: Human
Sample Types: Dried Blood Spot Sample (DBSS)
Applications: Luminex Development
Rosiglitazone treatment increases plasma levels of adiponectin and decreases levels of resistin in overweight women with PCOS: a randomized placebo-controlled study.
Authors: Majuri A, Santaniemi M, Rautio K, Kunnari A, Vartiainen J, Ruokonen A, Kesaniemi YA, Tapanainen JS, Ukkola O, Morin-Papunen L
Eur. J. Endocrinol., 2007-02-01;156(2):263-9.
Species: Human
Sample Types: Plasma
Applications: ELISA Development