Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Adiponutrin/PNPLA3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Adiponutrin/PNPLA3
Arg160-Arg349
Accession # Q9NST1

Specificity

Detects human Adiponutrin/PNPLA3 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Adiponutrin/PNPLA3 Antibody

Adiponutrin/ADPN antibody in Human Adipocytes by Immunocytochemistry (ICC).

Adiponutrin/ADPN in Human Adipocytes.

Adiponutrin/ADPN was detected in immersion fixed human adipocytes using 10 µg/mL Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Adiponutrin/ADPN antibody by Western Blot.

Detection of Human Adiponutrin/ADPN by Western Blot.

Western blot shows lysates of human adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Adiponutrin/ADPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5208) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adiponutrin/ADPN at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Adiponutrin/PNPLA3 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human adipocytes

Western Blot

1 µg/mL
Sample: Human adipose tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adiponutrin/PNPLA3

Human Adiponutrin (ADPN; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481 amino acids (aa) in length. It contains one patatin domain (aa 10‑179) that is associated with in vitro enzyme activity and a transmembrane segment between aa 42‑62. There appear to be two splice variants. One shows a four aa deletion of aa 59‑62 and a second shows an alternate start site at Met379. Over aa 160‑349, human ADPN shares 61% aa identity with mouse Adiponutrin.

Long Name

Patanin-like Phospholipase Domain-containing Protein 3

Alternate Names

ADPN, iPLA2-epsilon, PNPLA3

Entrez Gene IDs

80339 (Human); 362972 (Rat)

Gene Symbol

PNPLA3

UniProt

Q9NST1

Additional Adiponutrin/PNPLA3 Products

Product Documents for Human Adiponutrin/PNPLA3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Adiponutrin/PNPLA3 Antibody

For research use only

Citations for Human Adiponutrin/PNPLA3 Antibody

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Protocols

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