Human AdipoR1 Antibody

Catalog #: MAB10128 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB10128-100
MAB10128-SP

Detection of AdipoR1 in HEK293 Human Cell Line Transfected with Human AdipoR1 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AdipoR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human AdipoR1 in direct ELISAs. In direct ELISA, no cross-reactivity with human AdipoR2 is observed

Source

Recombinant Monoclonal Rabbit IgG₁ Clone # 2416C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing human AdipoR1 C-term sequences

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

3-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of AdipoR1 in HEK293 Human Cell Line Transfected with Human AdipoR1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human AdipoR1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human AdipoR1 Monoclonal Antibody (Catalog # MAB10128) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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AdipoR1 in Human Placenta. AdipoR1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human AdipoR1 Monoclonal Antibody (Catalog # MAB10128) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in decidual cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AdipoR1

Adiponectin receptor protein 1 (AdipoR1) is a 7 transmembrane receptor for globular and full-length adiponectin required for normal glucose and fat homeostasis and for maintaining a normal body weight. AdipoR1 mediates increased AMP kinase and PPAR-alpha ligand activities well as fatty-acid oxidation and glucose uptake by adiponectin. Genetic variability at the AdipoR1 locus is a strong determinant of coronary artery disease susceptibility in type 2 diabetes. AdipoR1 is abundantly expressed in skeletal muscle.

Long Name

Adiponectin Receptor 1

Entrez Gene IDs

51094 (Human); 72674 (Mouse); 289036 (Rat)

Alternate Names

ACDCR1; adiponectin receptor 1; adiponectin receptor protein 1; AdipoR1; ADR1; CGI45; FLJ42464; PAQR1; PAQR1FLJ25385; Progestin and adipoQ receptor family member I; TESBP1A