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Human AFAP Antibody

Catalog #: AF4558 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4558
AF4558-SP
Detection of Human AFAP by Western Blot.
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Human AFAP Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human AFAP in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human AFAP
His450-Thr730
Accession # Q8N556
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human AFAP antibody by Western Blot. View Larger

Detection of Human AFAP by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human AFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4558) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AFAP at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AFAP

AFAP (actin filament associated protein; also known as AFAP-110) is an adaptor protein that regulates actin filament integrity. It is both multimodular and multimeric, and is known to simultaneously bind actin and cytosolic kinases. As a homomultimer, AFAP binds and separates actin filaments, preventing actin cross-linking. Upon binding to kinases, AFAP is destabilized, promoting actin polymerization. Human AFAP is 730 amino acids (aa) in length, and contains SH3 and SH2 binding motifs (aa 69‑74 and aa 91‑96), two pleckstrin homology domains (aa 153‑248 and aa 347‑450) and a Leu-zipper/actin binding region (aa 511‑637). Over aa 450‑730, human AFAP is 86% identical to mouse and rat AFAP.

Long Name
Actin Filament-associated Protein, 110 kDa
Entrez Gene IDs
60312 (Human); 70292 (Mouse)
Alternate Names
actin filament associated protein 1; AFAP; AFAP1; AFAP-110; FLJ56849

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