Human Aggrecan (ADAMTS5-cleaved) Antibody

Catalog #: MAB64892
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB64892
MAB64892-SP

Detection of Human Aggrecan (ADAMTS5-cleaved) by Western Blot.

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All Aggrecan Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Aggrecan (ADAMTS5-cleaved) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Aggrecan in direct ELISAs. It specifically recognizes the N-terminal fragment of recombinant human Aggrecan released following cleavage by ADAMTS5.

Source

Monoclonal Mouse IgG_2B Clone # 725913

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLHG-conjugated human Aggrecan synthetic peptide
Leu384-Glu392
Accession # P16112

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Aggrecan (ADAMTS5-cleaved) by Western Blot. Western blot shows recombinant human Aggregan N-terminal and C-terminal fragments. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aggrecan Monoclonal Antibody (Catalog # MAB64892) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the Aggrecan N-terminal fragment at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aggrecan

Aggrecan, together with type II collagen, makes up to 90% of the dry weight of healthy cartilage. It hydrates the collagen network and thus provides cartilage with its properties of compressibility and elasticity. The N-terminal region of human Aggrecan (residues 20‑675) consists of two globular domains (G1 and G2), flanking an interglobular domain (IGD). The IGD contains the cleavage sites for matrix metalloproteinases and aggrecanases.

Entrez Gene IDs

176 (Human)

Alternate Names

ACAN; AGC1; AGC1SEDK; aggrecan core protein; Aggrecan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1; CSPG1aggrecan 1; CSPGCP; large aggregating proteoglycan; MSK16; MSK16AGCAN; SEDK

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Aggrecan (ADAMTS5-cleaved) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

CircSPI1_005 ameliorates osteoarthritis by sponging miR-370-3p to regulate the expression of MAP3K9
Authors: JL Zhou, S Deng, HS Fang, H Peng, QJ Hu
International immunopharmacology, 2022-07-20;110(0):109064.
Species: Mouse
Sample Types: Cell Lysate
Applications: Western Blot