Human AgRP/ART Antibody

Catalog #: AF704
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF704
AF704-SP

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All AgRP/ART Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AgRP/ART Antibody Summary

Species Reactivity

Human

Specificity

Detects human AgRP/ART in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse AgRP/ART is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human AgRP/ART
Ala21-Thr132
Accession # O00253

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human AgRP/ART (Catalog # 704-AG)

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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AgRP/ART in Human Brain. AgRP/ART was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF704) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown ; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AgRP/ART

Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuropeptide that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) on MC-3 and MC-4 receptors (1 - 4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature human AgRP is a 112 amino acid (aa) peptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Human AgRP shares 82% and 72% aa sequence identity with mouse and rat AgRP, respectively. It shares 32% aa sequence identity with Agouti. As in the case of Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones in a mechanism that does not involve melanocortin receptors (14).

References

Martin, N.M. et al. (2006) Peptides 27:333.
Fan, W. et al. (1997) Nature 385:165.
Ollmann, M.M. et al. (1997) Science 278:135.
Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
Shutter, J.R. et al. (1997) Genes Develop. 11:593.
Kiefer, L.L. et al. (1998) Biochemistry 37:991.
Jackson, P.J. et al. (2002) Biochemistry 41:7565.
Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
Small, C.K. et al. (2001) Diabetes 50:248.
Vink, T. et al. (2001) Mol. Psychiatry 6:325.
Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265 - 266:108.

Long Name

Agouti-related Protein

Entrez Gene IDs

181 (Human); 11604 (Mouse)

Alternate Names

agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2

Product Datasheets

Product Datasheet

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Citation for Human AgRP/ART Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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