Human AICL/CLEC-2B Antibody

Catalog #: MAB9059 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9059-100
MAB9059-SP

Detection of AICL/CLEC-2B in U937 cells by Flow Cytometry.

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Human AICL/CLEC-2B Antibody Summary

Species Reactivity

Human

Specificity

Detects human AICL/CLEC-2B in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1064935

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human AICL/CLEC-2B
Lys26-His149
Accession # Q92478

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

U937 human histiocytic lymphoma cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of AICL/CLEC-2B in U937 cells by Flow Cytometry. U937 cells were stained with Mouse Anti-Human AICL/CLEC-2B Monoclonal Antibody (Catalog # MAB9059, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AICL/CLEC-2B

Activation-induced C-type lectin (AICL), also known as CLEC2B, is a 30-35 kDa variably glycosylated type-2 transmembrane member of the C-type lectin-like receptor (CTLR) family. AICL belongs to the subgroup of CLEC2 proteins that also includes CLEC2A/KACL, CLEC2D/LLT, and CD69/CLEC2C, all of which are encoded by the natural killer gene complex (1). Human AICL contains a single C-type lectin domain in its extracellular region and a 7 amino acid cytoplasmic tail (2). AICL is expressed on monocytes, macrophages, and granulocytes (3), and it is upregulated on TLR-activated monocytes and IL-12 + IL-18 activated NK cells (3, 4). AICL is an activating receptor that triggers TNF production by monocytes (3). It binds to NKp80 on NK cells, resulting in NK cell mediated lysis of the AICL expressing monocyte (3). In addition, the AICL-NKp80 axis mediates interactions between activated and resting NK cells (4).

References

Li, Y. et al. (2014) Front. Immunol. 5:123.
Hamann, J. et al. (1997) Immunogenetics 45:295.
Welte, S. et al. (2006) Nat. Immunol. 7:1334.
Klimosch, S.N. et al. (2013) Blood 122:2380.

Long Name

Activation-Induced C-Type Lectin

Entrez Gene IDs

9976 (Human)

Alternate Names

Activation-induced C-type lectin; AICL; AICLC-type lectin superfamily member 2; CLEC2B; CLEC-2B; CLECSF2; CLECSF2IFN-alpha-2b-inducing-related protein 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 2 (activation-induced); C-type lectin domain family 2 member B; C-type lectin domain family 2, member B; HP10085; IFN-alpha2b-inducing related protein 1; IFNRG1