Human AIF-1/Iba1 Antibody Summary
Met1-Pro147
Accession # P55008
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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AIF-1/Iba1 in Human Spleen. AIF-1/Iba1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human AIF-1/Iba1 Monoclonal Antibody (Catalog # MAB7308) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AIF-1/Iba1
Allograft-inflammatory factor 1 (AIF-1),
also known as daintain and Iba1, is a 17 kDa cytoplasmic protein that contains
two calcium-binding EF hand motifs (aa 45-80 and aa 81-115). AIF-1 is expressed
in activated macrophages and is important for the proinflammatory response to
ischemia, tissue injury, and atherosclerosis. It is also expressed in vascular
smooth muscle and endothelial cells in these areas where it promotes vascular
remodeling and new vessel formation. AIF-1 is upregulated in allografted tissue
during transplant rejection. It also promotes the proliferation of breast
cancer cells and in vivo
tumorigenesis. The 147 amino acid (aa) isoform 3 of human AIF-1 shares 89% aa
sequence identity with mouse and rat AIF-1. Isoform 1 of human AIF-1 lacks the
N-terminal 54 aa of isoform 3. Isoform 2 lacks
aa 1-54 as well as aa 121-147
but also has a 66 aa insertion at position 65.
Product Datasheets
Citations for Human AIF-1/Iba1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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A molecular brain atlas reveals cellular shifts during the repair phase of stroke
Authors: Weber, RZ;Achon Buil, B;Rentsch, NH;Bosworth, A;Zhang, M;Kisler, K;Tackenberg, C;Zlokovic, BV;Rust, R;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Pyruvate kinase isoform M2 impairs cognition in systemic lupus erythematosus by promoting microglial synaptic pruning via the beta-catenin signaling pathway
Authors: L Lu, H Wang, X Liu, L Tan, X Qiao, J Ni, Y Sun, J Liang, Y Hou, H Dou
Journal of Neuroinflammation, 2021-10-13;18(1):229.
Species: Mouse
Sample Types: Whole Tissue
Applications: IF -
VSIG4 alleviates intracerebral hemorrhage induced brain injury by suppressing TLR4-regulated inflammatory response
Authors: D Zhang, X Shen, K Pang, Z Yang, A Yu
Brain research bulletin, 2021-08-19;176(0):67-75.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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1:1000 dilution is good
