Human ALCAM/CD166 Antibody

Catalog #: MAB6563 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB6563-100
MAB6563-SP

Detection of Human ALCAM/CD166 by Western Blot.

3 Images

All ALCAM/CD166 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ALCAM/CD166 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ALCAM/CD166 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2599C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # Q13740

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human ALCAM and eGFP

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

3-25 µg/mL

Immersion fixed A431 human epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human ALCAM/CD166 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ALCAM/CD166 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

View Larger

Detection of ALCAM in HEK293 Human Cell Line Transfected with Human ALCAM and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ALCAM or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human ALCAM Monoclonal Antibody (Catalog # MAB6563) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry

View Larger

ALCAM/CD166 in A431 Human Cell Line. ALCAM/CD166 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human ALCAM/CD166 Monoclonal Antibody (Catalog # MAB6563) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface.Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips Protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

References