Human ALK-1 Antibody Summary
Asp22-Gln118
Accession # P37023
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ALK-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALK-1 Monoclonal Antibody (Catalog # MAB370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALK-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human ALK‑1 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ALK‑1 at approximately 63 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human ALK‑1 Monoclonal Antibody (Catalog # MAB370). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ALK-1
Transforming Growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine- and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.
- ten Dijke, P. et al. (1993) Oncogene 8:2879.
- ten Dijke, P. et al. (1994) Science 264:101.
- Lux, A. et al. (1999) J. Biol. Chem. 274:9984.
Product Datasheets
Citations for Human ALK-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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ALK1 opposes ALK5/Smad3 signaling and expression of extracellular matrix components in human chondrocytes.
Authors: Finnson KW, Parker WL, ten Dijke P, Thorikay M, Philip A
J. Bone Miner. Res., 2008-06-01;23(6):896-906.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Antitumor activity of ALK1 in pancreatic carcinoma cells.
Authors: Ungefroren H, Schniewind B, Groth S, Chen WB, Muerkoster SS, Kalthoff H, Fandrich F
Int. J. Cancer, 2007-04-15;120(8):1641-51.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Therapeutic action of tranexamic acid in hereditary haemorrhagic telangiectasia (HHT): regulation of ALK-1/endoglin pathway in endothelial cells.
Authors: Fernandez-L A, Garrido-Martin EM, Sanz-Rodriguez F, Ramirez JR, Morales-Angulo C, Zarrabeitia R, Perez-Molino A, Bernabeu C, Botella LM
Thromb. Haemost., 2007-02-01;97(2):254-62.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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