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Human ALK-1 Antibody

Catalog #: AF370
18 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF370
AF370-SP
Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ALK-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ALK-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse ALK-1 is observed and less than 1% cross-reactivity with recombinant human (rh) Activin RIA and rhActivin RIB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALK‑1
Asp22-Gln118
Accession # P37023
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ALK‑1 Fc Chimera (Catalog # 370-AL)
Immunoprecipitation
David, L. et al. (2007) Blood. 109:1953.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-1

Transforming Growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine- and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

References
  1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  2. ten Dijke, P. et al. (1994) Science 264:101.
  3. Lux, A. et al. (1999) J. Biol. Chem. 274:9984.
Long Name
Activin Receptor-like Kinase 1
Entrez Gene IDs
94 (Human); 11482 (Mouse)
Alternate Names
activin A receptor type II-like 1; activin A receptor, type II-like kinase 1; Activin receptor-like kinase 1; ACVRL1; ACVRLK1ORW2; ALK1; ALK-1; ALK1TGF-B superfamily receptor type I; EC 2.7.11; HHT; HHT2EC 2.7.11.30; serine/threonine-protein kinase receptor R3; SKR3; TSR-I

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Citations for Human ALK-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. High concentrations of soluble endoglin can inhibit BMP9 signaling in non-endothelial cells
    Authors: C Andersson-, B Liu, I Quist-Løkk, PD Upton, OE Olsen, H Hella, X Yang, Z Tong, NW Morrell, T Holien, W Li
    Scientific Reports, 2023-04-24;13(1):6639.
  2. Elevated BMPR2 expression amplifies osteoblast differentiation in ankylosing spondylitis
    Authors: Sungsin Jo, Seung Hoon Lee, Chanhyeok Jeon, Hye-Ryeong Jo, Eunae Ko, Min Whangbo et al.
    Journal of Rheumatic Diseases
  3. Computational and Experimental Analyses for Pathogenicity Prediction of ACVRL1 Missense Variants in Hereditary Hemorrhagic Telangiectasia
    Authors: Iwasa, T;Urasaki, A;Kakihana, Y;Nagata-Akaho, N;Harada, Y;Takeda, S;Kawamura, T;Shiraishi, I;Kurosaki, K;Morisaki, H;Yamada, O;Nakagawa, O;
    Journal of clinical medicine
    Species: Mouse
    Sample Types: Transfected Whole Cells
    Applications: Immunocytochemistry
  4. WNK1 collaborates with TGF-beta in endothelial cell junction turnover and angiogenesis
    Authors: AB Jaykumar, S Plumber, DM Barry, D Binns, C Wichaidit, M Grzemska, S Earnest, EJ Goldsmith, O Cleaver, MH Cobb
    Proceedings of the National Academy of Sciences of the United States of America, 2022-07-22;119(30):e2203743119.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells
    Authors: B Tao, JR Kraehling, S Ghaffari, CM Ramirez, S Lee, JW Fowler, WL Lee, C Fernandez-, A Eichmann, WC Sessa
    J Biol Chem, 2020-10-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Decreased Colonic Activin Receptor-Like Kinase 1 Disrupts Epithelial Barrier Integrity in Patients with Crohn's disease
    Authors: T Toyonaga, EC Steinbach, BP Keith, JB Barrow, MR Schaner, EA Wolber, C Beasley, J Huling, Y Wang, NL Allbritton, N Chaumont, TS Sadiq, MJ Koruda, A Jain, MD Long, EL Barnes, HH Herfarth, KL Isaacs, JJ Hansen, MT Shanahan, R Rahbar, TS Furey, P Sethupathy, SZ Sheikh
    Cell Mol Gastroenterol Hepatol, 2020-06-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. BMP9 signaling promotes the normalization of tumor blood vessels
    Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
    Oncogene, 2020-02-10;0(0):.
    Species: Transgenic Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. BMP9 (Bone Morphogenetic Protein-9)/Alk1 (Activin-Like Kinase Receptor Type I) Signaling Prevents Hyperglycemia-Induced Vascular Permeability
    Authors: Naoufal Akla, Claire Viallard, Natalija Popovic, Cindy Lora Gil, Przemyslaw Sapieha, Bruno Larrivée
    Arteriosclerosis, Thrombosis, and Vascular Biology
  9. Defective fluid shear stress mechanotransduction mediates hereditary hemorrhagic telangiectasia
    Authors: Nicolas Baeyens, Bruno Larrivée, Roxana Ola, Brielle Hayward-Piatkowskyi, Alexandre Dubrac, Billy Huang et al.
    Journal of Cell Biology
  10. Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension.
    Authors: Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S
    PLoS ONE, 2014-06-23;9(6):e100310.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
    Authors: van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
    J. Biol. Chem., 2012-04-05;287(22):18551-61.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  12. Functional analysis of the BMP9 response of ALK1 mutants from HHT2 patients: a diagnostic tool for novel ACVRL1 mutations.
    Authors: Ricard N, Bidart M, Mallet C
    Blood, 2010-05-25;116(9):1604-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Endoglin and activin receptor-like-kinase 1 are co-expressed in the distal vessels of the lung: implications for two familial vascular dysplasias, HHT and PAH.
    Authors: Mahmoud M, Borthwick GM, Hislop AA, Arthur HM
    Lab. Invest., 2008-11-17;89(1):15-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production.
    Authors: Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L
    Am. J. Pathol., 2008-03-18;172(4):1127-40.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  15. Activin receptor-like kinase 1 inhibits human microvascular endothelial cell migration: potential roles for JNK and ERK.
    Authors: David L, Mallet C, Vailhe B, Lamouille S, Feige JJ, Bailly S
    J. Cell. Physiol., 2007-11-01;213(2):484-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Cyclic stretch controls the expression of CD40 in endothelial cells by changing their transforming growth factor-beta1 response.
    Authors: Korff T, Aufgebauer K, Hecker M
    Circulation, 2007-10-29;116(20):2288-97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. The transforming growth factor-beta/Smad2,3 signalling axis is impaired in experimental pulmonary hypertension.
    Authors: Zakrzewicz A, Kouri FM, Nejman B, Kwapiszewska G, Hecker M, Sandu R, Dony E, Seeger W, Schermuly RT, Eickelberg O, Morty RE
    Eur. Respir. J., 2007-03-28;29(6):1094-104.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells.
    Authors: David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S
    Blood, 2006-10-26;109(5):1953-61.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Human ALK-1 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: Calu-6 human lung carcinoma cell line,HCC827 human non-small cell lung cancer cell line Species: Human
Western Blot Human ALK-1 Antibody AF370