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Human ALK-7 Antibody

Catalog #: MAB77491
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB77491
MAB77491-SP
Detection of ALK‑7 in PC‑3 Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ALK-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ALK‑7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat ALK-7 is observed.
Source
Monoclonal Mouse IgG2B Clone # 810506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ALK‑7
Leu26-Glu113
Accession # Q8NER5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ALK-7 antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ALK‑7 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human ALK-7 Monoclonal Antibody (Catalog # MAB77491, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-7

Activin receptor-like kinase 7 (ALK-7), also known as Activin R1C (gene name ACVR1C), is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. It associates with type II receptors to form a signaling complex that responds to the ligands Activin AB, and Activin B, GDF3, and Nodal. ALK-7 plays a role in regulating energy balance by inhibiting insulin secretion and inducing pancreatic beta cell apoptosis. It is expressed in adipose tissue but downregulated in obesity. ALK-7 is also expressed in pituitary gonadotropic cells and in pre-eclamptic placenta. It induces the apoptosis of trophoblasts as well as ovarian granulosa and epithelial cells. Within the extracellular domain, human ALK-7 shares 95% and 91% amino acid (aa) sequence identity with mouse and rat ALK-7, respectively. Alternate splicing of human ALK-7 generates additional isoforms with either a 50 aa N-terminal truncation or with deletions of 79 aa or 157 aa that encompass the transmembrane segment.

Long Name
Activin Receptor-like Kinase 7
Entrez Gene IDs
130399 (Human); 245921 (Rat)
Alternate Names
activin A receptor, type IC; Activin receptor type IC; activin receptor type-1C; Activin receptor-like kinase 7; Activin RIC; ACTR-IC; ACVR1C; ACVRLK7; ALK7; ALK-7; ALK7ALK-7; EC 2.7.11; EC 2.7.11.30

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Citations for Human ALK-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer
    Authors: T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi
    Oncol Rep, 2020-10-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs
    Authors: Helmout Modjtahedi
    Int. J. Oncol., 2016-09-05;49(5):1825-1838.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot

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