Home / Alkaline Phosphatase/ALPL / Human Alkaline Phosphatase/ALPL Antibody

Human Alkaline Phosphatase/ALPL Antibody

Catalog #: MAB2909
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2909
MAB2909-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Alkaline Phosphatase/ALPL Antibody Summary

Species Reactivity
Human
Specificity
Detects human Alkaline Phosphatase/ALPL in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 388816
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Alkaline Phosphatase/ALPL
Leu18-Ser502
Accession # P05186
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Alkaline Phosphatase/ALPL (Catalog # 2909-AP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPL

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.

References
  1. Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
  2. Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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Citations for Human Alkaline Phosphatase/ALPL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. p53 Regulates Mitochondrial Dynamics in Vascular Smooth Muscle Cell Calcification
    Authors: K Phadwal, QY Tang, I Luijten, JF Zhao, B Corcoran, RK Semple, IG Ganley, VE MacRae
    International Journal of Molecular Sciences, 2023-01-13;24(2):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Ablation of Enpp6 Results in Transient Bone Hypomineralization
    Authors: Scott Dillon, Karla Suchacki, Shun‐Neng Hsu, Louise A Stephen, Rongling Wang, William P Cawthorn et al.
    JBMR Plus
  3. Increased activity of TNAP compensates for reduced adenosine production and promotes ectopic calcification in the genetic disease ACDC
    Authors: Hui Jin, Cynthia St. St. Hilaire, Yuting Huang, Dan Yang, Natalia I. Dmitrieva, Alejandra Negro et al.
    Science Signaling
  4. Vascular calcification is dependent on plasma levels of pyrophosphate.
    Authors: Lomashvili K, Narisawa S, Millan J, O'Neill W
    Kidney Int, 2014-04-09;85(6):1351-6.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
    Authors: Deschaseaux F, Gaillard J, Langonne A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensebe L
    FASEB J, 2013-04-16;27(8):2977-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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