Human Alkaline Phosphatase/ALPL Antibody Summary
Leu18-Ser502
Accession # P05186
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Alkaline Phosphatase/ALPL in BG01V Human Embryonic Stem Cells. Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503‑524) is not present in the mature form.
- Le Du, M-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808.
- Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1.
- Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Product Datasheets
Citations for Human Alkaline Phosphatase/ALPL Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Tissue Nonspecific Alkaline Phosphatase Function in Bone and Muscle Progenitor Cells: Control of Mitochondrial Respiration and ATP Production
Authors: Z Zhang, HK Nam, S Crouch, NE Hatch
International Journal of Molecular Sciences, 2021-01-24;22(3):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis
Authors: R Fu, WC Lv, Y Xu, MY Gong, XJ Chen, N Jiang, Y Xu, QQ Yao, L Di, T Lu, LM Wang, R Mo, ZQ Wu
Nat Commun, 2020-01-23;11(1):460.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency.
Authors: Onishi K, Tonge P, Nagy A, Zandstra P
Stem Cell Reports, 2014-06-06;3(1):156-68.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Enzyme replacement prevents enamel defects in hypophosphatasia mice.
Authors: Yadav MC, de Oliveira RC, Foster BL, Fong H, Cory E, Narisawa S, Sah RL, Somerman M, Whyte MP, Millan JL
J. Bone Miner. Res., 2012-08-01;27(8):1722-34.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Central role of pyrophosphate in acellular cementum formation.
Authors: Foster BL, Nagatomo KJ, Nociti FH, Fong H, Dunn D, Tran AB, Wang W, Narisawa S, Millan JL, Somerman MJ
PLoS ONE, 2012-06-04;7(6):e38393.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Therapeutic Antibody Targeting Tumor- and Osteoblastic Niche-Derived Jagged1 Sensitizes Bone Metastasis to Chemotherapy
Authors: Hanqiu Zheng, Yangjin Bae, Sabine Kasimir-Bauer, Rebecca Tang, Jin Chen, Guangwen Ren et al.
Cancer Cell
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Antibody staining was performed on mouse bone marrow sections. Please refer to PMID 29232552 for this staining, experimental details, and other data using this antibody.
