Human Alkaline Phosphatase/ALPL Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).
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Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline Phosphatase/ALPL and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Alkaline Phosphatase/ALPL in Human Kidney. Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Alkaline Phosphatase/ALPL in Whole blood granulocytes by Flow Cytometry. Whole blood granulocytes were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human Alkaline Phosphatase/ALPL by Immunocytochemistry/Immunofluorescence Immunocytochemistry of pluripotency markers.Staining was performed 48 hours following the last passage. From left to right: staining displayed by hESCs (HS181 line, low and high magnifications), hNPCs, v-myc immortalized lines (hNS1, hVM1, and hCTX) and fibroblasts (hFF-1). From top to bottom the markers studied were: NANOG, OCT3/4, SOX2 and AP. Hoechst 33258 nuclear staining is shown in blue. Scale bar represents 100 μm for hESCs in the left hand column (low magnification) and 25 μm for all the other microphotographs. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0118499), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Alkaline Phosphatase/ALPL in iPSCs by Flow Cytometry iPSCs were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.
- Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
- Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
- Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Product Datasheets
Citations for Human Alkaline Phosphatase/ALPL Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Platelet lysate for expansion or osteogenic differentiation of bone marrow mesenchymal stem cells for 3D tissue constructs
Authors: Anerillas, LO;Wiberg, M;Kingham, PJ;Kelk, P;
Regenerative therapy
Species: Human
Sample Types: Whole Cells
Applications: Immunohistochemistry -
ALPL-1 is a target for chimeric antigen receptor therapy in osteosarcoma
Authors: Mensali, N;Köksal, H;Joaquina, S;Wernhoff, P;Casey, NP;Romecin, P;Panisello, C;Rodriguez, R;Vimeux, L;Juzeniene, A;Myhre, MR;Fåne, A;Ramírez, CC;Maggadottir, SM;Duru, AD;Georgoudaki, AM;Grad, I;Maturana, AD;Gaudernack, G;Kvalheim, G;Carcaboso, AM;de Alava, E;Donnadieu, E;Bruland, ØS;Menendez, P;Inderberg, EM;Wälchli, S;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: ICC -
BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D In Vitro Models
Authors: S Martin-Igl, L Milian, M Sancho-Tel, R Salvador-C, JJ Martín de, C Carda, M Mata
Stem Cells International, 2022-03-04;2022(0):4910399.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition
Authors: Liangcong Hu, Jing Liu, Hang Xue, Adriana C. Panayi, Xudong Xie, Ze Lin et al.
Molecular Therapy - Nucleic Acids
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Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells
Authors: J Camps, H Grosemans, R Gijsbers, C Maes, M Sampaolesi
Int J Mol Sci, 2019-03-05;20(5):.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
Authors: Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A
PLoS ONE, 2015-03-12;10(3):e0118499.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Osteodifferentiated mesenchymal stem cells from bone marrow and adipose tissue express HLA-G and display immunomodulatory properties in HLA-mismatched settings: implications in bone repair therapy.
Authors: Montespan F, Deschaseaux F, Sensebe L, Carosella E, Rouas-Freiss N
J Immunol Res, 2014-04-30;2014(0):230346.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: Flow Cytometry -
Immunohistochemical expression of hard tissue related factors in the mouse dental pulp after immediate teeth separation
Authors: A Nabeyama, K Nakano, S Saito, M sato, N Okafuji, A Yamamoto et al.
European Journal of Medical Research
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Cementum- and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig's epithelial root sheath cells.
Authors: Bai Y, Bai Y, Matsuzaka K, Hashimoto S, Fukuyama T, Wu L, Miwa T, Liu X, Wang X, Inoue T
Bone, 2011-03-01;48(6):1417-26.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro.
Authors: Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS
Bone, 2009-04-18;45(2):309-20.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
In vivo impact of a 4 bp deletion mutation in the DLX3 gene on bone development.
Authors: Choi SJ, Roodman GD, Feng JQ, Song IS, Amin K, Hart PS, Wright JT, Haruyama N, Hart TC
Dev. Biol., 2008-10-25;325(1):129-37.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold.
Authors: Valarmathi MT, Yost MJ, Goodwin RL, Potts JD
Biomaterials, 2008-03-04;29(14):2203-16.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Human embryonic and neuronal stem cell markers in retinoblastoma
Authors: Gail M. Seigel, Abigail S. Hackam, Arupa Ganguly, Lorrie M. Mandell, Federico Gonzalez-Fernandez
Mol Vis
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Bone marrow-derived osteoblast progenitor cells in circulating blood contribute to ectopic bone formation in mice.
Authors: Otsuru S, Tamai K, Yamazaki T, Yoshikawa H, Kaneda Y
Biochem. Biophys. Res. Commun., 2007-01-10;354(2):453-8.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption
Authors: Seiya Kanno, Yusuke Okubo, Tatsuto Kageyama, Lei Yan, Satoshi Kitajima, Junji Fukuda
iScience
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