Human Alkaline Phosphatase/
ALPL Biotinylated Antibody
Human Alkaline Phosphatase/
ALPL Biotinylated Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Alkaline Phosphatase/ALPL in Human Osteoblasts. Alkaline Phosphatase/ALPL was detected in immersion fixed differentiated human osteoblasts using Human Alkaline Phosphatase/ALPL Biotinylated Monoclonal Antibody (Catalog # BAM1448) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.
- Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
- Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
- Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Product Datasheets
Citations for Human Alkaline Phosphatase/
ALPL Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Activation of cancer-related and mitogen-activated protein kinase signaling pathways in human mature osteoblasts isolated from patients with type 2 diabetes
Authors: T Kuroiwa, M Matsumoto, R Kato, A Nimura, T Yoshii, A Okawa, K Fujita
Bone Rep, 2019-02-21;10(0):100199.
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection -
Effects of intermittent parathyroid hormone treatment on osteoprogenitor cells in postmenopausal women.
Authors: Drake MT, Srinivasan B, Modder UI, Ng AC, Undale AH, Roforth MM, Peterson JM, McCready LK, Riggs BL, Khosla S
Bone, 2011-05-11;49(3):349-55.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.
Authors: Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P
Biomaterials, 2009-04-10;30(20):3415-27.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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Reconstitution Buffers
Staining Reagents
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Reviews for Human Alkaline Phosphatase/
ALPL Biotinylated Antibody
Average Rating: 4 (Based on 1 Review)
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ALPL Biotinylated Antibody?
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