Human alpha-1B Adrenergic R/ADRA1B Antibody

Catalog #: MAB4730 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4730
MAB4730-SP

Detection of ADRA1B in PC‑3 Human Cell Line by Flow Cytometry.

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All alpha-1B Adrenergic R/ADRA1B Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-1B Adrenergic R/ADRA1B Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADRA1B. Stains human ADRA1B transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 471802

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human ADRA1B
Met1-Phe520
Accession # P35368

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of ADRA1B antibody in PC-3 Human Cell Line antibody by Flow Cytometry.

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Detection of ADRA1B in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human ADRA1B Monoclonal Antibody (Catalog # MAB4730, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-1B Adrenergic R/ADRA1B

ADRA1B, also known as the alpha -1B-adrenergic receptor, is a 90 kDa 7TM catecholamine receptor with selectivity for epinephrine and norepinephrine. Formation of homooligomers and heterooligomers with ADRA1A enables receptor internalization following ligand binding. ADRA1B functions in diverse settings including vasoconstriction and myocardial contractility, neuronal dopaminergic responses, dendritic cell migration and inflammatory responses, and neuroendocrine regulation of fertility. Human ADRA1B shares 95% amino acid sequence identity with mouse and rat ADRA1B.

Long Name

Alpha-1B Adrenergic Receptor

Entrez Gene IDs

147 (Human); 11548 (Mouse); 24173 (Rat)

Alternate Names

a-1B AdrenergicR; ADRA1; ADRA1B; adrenergic, alpha-1B-, receptor; alpha-1B Adrenergic R; alpha-1B adrenergic receptor; alpha1B AdrenergicR; Alpha-1B adrenoceptor; Alpha-1B adrenoreceptor; alpha-1B-adrenergic receptor; ALPHA1BAR