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Human alpha 2-Macroglobulin Antibody

Catalog #: MAB19381 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB19381-100
MAB19381-SP
Human alpha 2‑Macroglobulin ELISA Standard Curve.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha 2-Macroglobulin Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha 2-Macroglobulin in direct ELISA, this antibody does not cross-react with rhC5a or rmC5a.
Source
Monoclonal Mouse IgG1 Clone # 257303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human plasma-derived alpha 2-Macroglobulin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human alpha 2‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha 2-Macroglobulin DuoSet ELISA Kit (Catalog # DY1938) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA View Larger

Human alpha 2‑Macroglobulin ELISA Standard Curve. Recombinant Human alpha 2‑Macroglobulin protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha 2‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 2-Macroglobulin

Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.

Entrez Gene IDs
2 (Human)
Alternate Names
A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7

Product Datasheets

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