Human alpha-Internexin Antibody

Catalog #: MAB4844 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4844
MAB4844-SP

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Detection of Human alpha -Internexin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-Internexin Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha ‑Internexin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NF-M or rhVimentin is observed.

Source

Monoclonal Mouse IgG₁ Clone # 529724

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human alpha -Internexin
Val230-Glu450
Accession # Q16352

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

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Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human alpha -Internexin by Western Blot. Western blot shows lysates of human cortex tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a-Internexin Monoclonal Antibody (Catalog # MAB4844) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for a-Internexin at approximately 50-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Internexin

alpha -Internexin ( alpha -Inx; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha -Inx is one of four Class IV neuronal IF proteins. It both self-assembles and complexes with NF-L, H and M in select cortical and cerebellar neurons. alpha -Inx contains one DNA-binding region (aa 10‑92), a poly‑Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha -Inx shows more that 96% aa identity with mouse, canine and rat alpha -Inx.

Entrez Gene IDs

9118 (Human); 226180 (Mouse); 24503 (Rat)

Alternate Names

66 kDa neurofilament protein; alphaInternexin; alpha-Internexin; alpha-Inx; FLJ18662; FLJ57501; INA; internexin neuronal intermediate filament protein, alpha; MGC12702; NEF5; neurofilament 5 (66kD); Neurofilament 5; Neurofilament-66; neurofilament-66, tax-binding protein; NF-66; NF-66alpha-internexin; TXBP-1