Human alpha -Synuclein Antibody

Catalog #: MAB11376 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11376-100
MAB11376-SP

Detection of alpha -Synuclein in SK‑Mel‑28 cells (positive) and HL‑60 cells (negative).

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha -Synuclein Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha -Synuclein in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1060506

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived human alpha-Synuclein
Met1-Ala140
Accession # P37840

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Brain Cerebellum

Immunocytochemistry

5-25 µg/mL

Immersion fixed SK‑Mel‑28 human malignant melanoma cells (positive) and HL‑60 human acute promyelocytic leukemia cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of alpha -Synuclein in SK‑Mel‑28 cells (positive) and HL‑60 cells (negative). alpha -Synuclein was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cells (positive) and absent in HL‑60 human acute promyelocytic leukemia cells (negative) using Mouse Anti-Human alpha -Synuclein Monoclonal Antibody (Catalog # MAB11376) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Detection of alpha -Synuclein in Human Brain Cerebellum. alpha -Synuclein was detected in immersion fixed paraffin-embedded sections of Human Brain Cerebellum using Mouse Anti-Human alpha -Synuclein Monoclonal Antibody (Catalog # MAB11376) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to medulla. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Synuclein

alpha -Synuclein is member of a family of small soluble proteins that include also beta -, and gamma -Synuclein. It is predominantly expressed in neurons of the central nervous system in the presynaptic region of nerve terminals, where it cycles between a free, partially unfolded and a helical, membrane-bound form. alpha -Synuclein can self-aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms of alpha -Synuclein are major components of Lewy bodies and Lewy neurites and have been linked to the pathogenesis of Parkinson’s Disease, Parkinson’s Disease Dementia, and dementia with Lewy bodies. alpha -Synuclein aggregates can be also found associated with amyloid plaques in Alzheirmer’s Disease.

References

Breydo L. et al. (2012) Biochim. Biophys. Acta. 1822:261.
Chen R.H. et al. (2013) J. Biol. Chem. 288:7438.
Li X. et al. (2008) Acta Biochim. Biophys. Sin (Shanghai) 40:406.
Surguchov A. (2008) Int. Rev. Cell Mol. Biol. 270:225.
Xia Q. et al. (2008) Front. Biosci. 13:3850.

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Alternate Names

alpha-Synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha; truncated alpha synuclein