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Human AMIGO2 Antibody

Catalog #: MAB2080
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2080
MAB2080-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AMIGO2 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human AMIGO2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human AMIGO1, recombinant mouse (rm) AMIGO2, or rmAMIGO3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 273617
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human AMIGO2
Gly39-Ala392
Accession # Q86SJ2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human AMIGO2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO2

AMIGO2 is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (LRRs) and one Ig domain in the extracellular region. AMIGO2 is expressed primarily in cerebellum, retina, liver, and lung. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties.

Long Name
Amphoterin-induced Gene and ORF 2
Entrez Gene IDs
347902 (Human); 105827 (Mouse); 102124161 (Cynomolgus Monkey)
Alternate Names
adhesion molecule with Ig-like domain 2; ALI1amphoterin induced gene 2; alivin 1; Alivin-1; AMIGO2; AMIGO-2; DEGAamphoterin-induced protein 2; differentially expressed in gastric adenocarcinoma; Differentially expressed in gastric adenocarcinomas; transmembrane protein AMIGO2

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Citation for Human AMIGO2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expression profile of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins during skin barrier repair.
    Authors: de Koning HD, van den Bogaard EH, Bergboer JG, Kamsteeg M, van Vlijmen-Willems IM, Hitomi K, Henry J, Simon M, Takashita N, Ishida-Yamamoto A, Schalkwijk J, Zeeuwen PL
    Br. J. Dermatol., 2012-06-01;166(6):1245-54.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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