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Human Aminoacylase/ACY1 Antibody

Catalog #: MAB2900
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2900
MAB2900-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Aminoacylase/ACY1 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human Aminoacylase/ACY1 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse ACY1 is observed.
Source
Monoclonal Rat IgG2B Clone # 475626
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminoacylase/ACY1
Thr2-Ser408
Accession # Q03154
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Aminoacylase/ACY1 (Catalog # 2900-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Aminoacylase/ACY1 (Catalog # 2900-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminoacylase/ACY1

The human ACY1 gene encodes Aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active as described in Activity Assay Protocol.

References
  1. Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
  2. Maceyka, M. et al. (2004) FEBS Lett. 568:30.
Entrez Gene IDs
95 (Human); 109652 (Mouse)
Alternate Names
ACY1; ACY-1; ACY1D; acylase; aminoacylase 1; Aminoacylase; aminoacylase-1; EC 3.5.1; EC 3.5.1.14; N-acyl-L-amino-acid amidohydrolase

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Citation for Human Aminoacylase/ACY1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Serum aminoacylase-1 is a novel biomarker with potential prognostic utility for long-term outcome in patients with delayed graft function following renal transplantation.
    Authors: Welberry Smith, Matthew, Zougman, Alexandr, Cairns, David A, Wilson, Michelle, Wind, Tobias, Wood, Steven L, Thompson, Douglas, Messenger, Michael, Mooney, Andrew, Selby, Peter J, Lewington, Andrew J, Banks, Rosamond
    Kidney Int, 2013-06-05;84(6):1214-25.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)

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