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Human Amyloid beta (aa1-42) Quantikine ELISA Kit

Catalog #: DAB142
36 Citations Datasheet / COA / SDS
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DAB142
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Human Amyload beta ELISA Standard Curve
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Citations (36)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Amyloid beta (aa1-42) Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity
4.73 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
Specificity
Natural human Amyloid beta (aa1-42).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Amyloid beta (aa1-42) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-42) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-42) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta
(aa1-42) showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-42).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 46.3 124 264 48.9 136 274
Standard Deviation 2.46 4.4 9.38 3.48 9.02 16.1
CV% 5.3 3.5 3.6 7.1 6.6 5.9

Recovery

The recovery of human Amyloid beta (aa1-42) spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 82-114
Cell Lysis Buffer (n=2) 92 90-94
Cerebrospinal Fluid (n=4) 107 95-120

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta (aa1-42) were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
Human Amyload beta ELISA Linearity

Scientific Data

N/A Human Amyload beta ELISA Standard Curve View Larger

Human Amyload beta ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amyloid beta

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Entrez Gene IDs:
351 (Human); 11820 (Mouse)
Alternate Names:
Abeta; Amyloid beta; beta-Amyloid Peptide
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Amyloid beta (aa1-42) Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Alzheimer's disease protective allele of Clusterin modulates neuronal excitability through lipid-droplet-mediated neuron-glia communication
    Authors: Zhao, X;Li, Y;Zhang, S;Sudwarts, A;Zhang, H;Kozlova, A;Moulton, MJ;Goodman, LD;Pang, ZP;Sanders, AR;Bellen, HJ;Thinakaran, G;Duan, J;
    medRxiv : the preprint server for health sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  2. A new neuroprotective candidate TJ1 targeting amyloidogenesis in 5xFAD Alzheimer's disease mice
    Authors: Deng, JL;Huang, LF;Bian, ZY;Feng, XY;Qi, RY;Dong, WX;Gao, JM;Tang, JJ;
    International immunopharmacology
    Species: Rat, Transgenic Mouse
    Sample Types: Tissue Homogenates
  3. Microglia-targeted inhibition of miR-17 via mannose-coated lipid nanoparticles improves pathology and behavior in a mouse model of Alzheimer's disease
    Authors: Badr, A;Daily, KP;Eltobgy, M;Estfanous, S;Tan, MH;Chun-Tien Kuo, J;Whitham, O;Carafice, C;Gupta, G;Amer, HM;Shamseldin, MM;Yousif, A;Deems, NP;Fitzgerald, J;Yan, P;Webb, A;Zhang, X;Pietrzak, M;Ghoneim, HE;Dubey, P;Barrientos, RM;Lee, RJ;Kokiko-Cochran, ON;Amer, AO;
    Brain, behavior, and immunity
    Species: Mouse, Transgenic Mouse
    Sample Types: Tissue Homogenates
  4. A single nuclear transcriptomic characterisation of mechanisms responsible for impaired angiogenesis and blood-brain barrier function in Alzheimer's disease
    Authors: Tsartsalis, S;Sleven, H;Fancy, N;Wessely, F;Smith, AM;Willumsen, N;Cheung, TKD;Rokicki, MJ;Chau, V;Ifie, E;Khozoie, C;Ansorge, O;Yang, X;Jenkyns, MH;Davey, K;McGarry, A;Muirhead, RCJ;Debette, S;Jackson, JS;Montagne, A;Owen, DR;Miners, JS;Love, S;Webber, C;Cader, MZ;Matthews, PM;
    Nature communications
    Species: Human
    Sample Types: Cell Extract
  5. SELENOK-dependent CD36 palmitoylation regulates microglial functions and A? phagocytosis
    Authors: Ouyang, P;Cai, Z;Peng, J;Lin, S;Chen, X;Chen, C;Feng, Z;Wang, L;Song, G;Zhang, Z;
    Redox biology
    Species: Mouse
    Sample Types: Bacteria
  6. Circuit Training Improves the Levels of ?-Amyloid and Brain-Derived Neurotrophic Factor Related to Cognitive Impairment Risk Factors in Obese Elderly Korean Women
    Authors: Kang, D;Koh, S;Kim, T;Bressel, E;Kim, D;
    Journal of clinical medicine
    Species: Human
    Sample Types: Serum
  7. Amyloid-? specific regulatory T cells attenuate Alzheimer's disease pathobiology in APP/PS1 mice
    Authors: Yeapuri, P;Machhi, J;Lu, Y;Abdelmoaty, MM;Kadry, R;Patel, M;Bhattarai, S;Lu, E;Namminga, KL;Olson, KE;Foster, EG;Mosley, RL;Gendelman, HE;
    Molecular neurodegeneration
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Adipose tissue coregulates cognitive function
    Authors: Oliveras-Cañellas, N;Castells-Nobau, A;de la Vega-Correa, L;Latorre-Luque, J;Motger-Albertí, A;Arnoriaga-Rodriguez, M;Garre-Olmo, J;Zapata-Tona, C;Coll-Martínez, C;Ramió-Torrentà, L;Moreno-Navarrete, JM;Puig, J;Villarroya, F;Ramos, R;Casadó-Anguera, V;Martín-García, E;Maldonado, R;Mayneris-Perxachs, J;Fernández-Real, JM;
    Science advances
  9. MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice
    Authors: Ge, J;Xue, Z;Shu, S;Yu, L;Qin, R;Tao, W;Liu, P;Dong, X;Lan, Z;Bao, X;Ye, L;Xu, Y;Zhu, X;
    JCI insight
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Selective PPAR-Delta/PPAR-Gamma Activation Improves Cognition in a Model of Alzheimer's Disease
    Authors: Steinke, I;Govindarajulu, M;Pinky, PD;Bloemer, J;Yoo, S;Ward, T;Schaedig, T;Young, T;Wibowo, FS;Suppiramaniam, V;Amin, RH;
    Cells
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Machine-learning-based analysis of the sensitivity and specificity on lipid-lowering effect of one-month-administered statins
    Authors: H Liu, R Jiao, L Wang, F Feng, X Zhao, J Yang
    Medicine, 2023-03-03;102(9):e33139.
    Species: Human
    Sample Types: Serum
  12. Role of the Caspase-8/RIPK3 axis in Alzheimer's disease pathogenesis and Abeta-induced NLRP3 inflammasome activation
    Authors: S Kumar, S Budhathoki, CB Oliveira, AD Kahle, OY Calhan, JR Lukens, CD Deppmann
    JCI Insight, 2023-02-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. P. gingivalis-LPS Induces Mitochondrial Dysfunction Mediated by Neuroinflammation through Oxidative Stress
    Authors: A Verma, G Azhar, X Zhang, P Patyal, G Kc, S Sharma, Y Che, JY Wei
    International Journal of Molecular Sciences, 2023-01-04;24(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Human PSEN1 Mutant Glia Improve Spatial Learning and Memory in Aged Mice
    Authors: H Jäntti, M Oksanen, P Kettunen, S Manta, L Mouledous, H Koivisto, J Ruuth, K Trontti, H Dhungana, M Keuters, I Weert, M Koskuvi, I Hovatta, AM Linden, C Rampon, T Malm, H Tanila, J Koistinaho, T Rolova
    Cells, 2022-12-18;11(24):.
    Species: Human
    Sample Types: Tissue Homogenates
  15. Potential Therapeutic Activity of Berberine in Thyroid-Associated Ophthalmopathy: Inhibitory Effects on Tissue Remodeling in Orbital Fibroblasts
    Authors: J Diao, X Chen, P Mou, X Ma, R Wei
    Investigative Ophthalmology & Visual Science, 2022-09-01;63(10):6.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. An in vitro workflow of neuron-laden agarose-laminin hydrogel for studying small molecule-induced amyloidogenic condition
    Authors: P Namchaiw, P Bunreangsr, P Eiamcharoe, S Eiamboonse, R P Poo-Arpo
    PLoS ONE, 2022-08-26;17(8):e0273458.
    Species: Human
    Sample Types: Cell Lysates
  17. Blood circulating miR-28-5p and let-7d-5p associate with premature ageing in Down syndrome
    Authors: C Morsiani, MG Bacalini, S Collura, M Moreno-Vil, N Breusing, A Bürkle, T Grune, C Franceschi, M De Eguileo, M Capri
    Mechanisms of Ageing and Development, 2022-06-30;206(0):111691.
    Species: Human
    Sample Types: Plasma
  18. High frequency repetitive transcranial magnetic stimulation alleviates cognitive deficits in 3xTg-AD mice by modulating the PI3K/Akt/GLT-1 axis
    Authors: H Cao, C Zuo, Z Gu, Y Huang, Y Yang, L Zhu, Y Jiang, F Wang
    Redox Biology, 2022-05-30;54(0):102354.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Recurrent moderate hypoglycemia accelerates the progression of cognitive deficits through impairment of TRPC6/GLUT3 pathway in diabetic APP/PS1 mice
    Authors: C He, Q Li, Y Cui, P Gao, W Shu, Q Zhou, L Wang, L Li, Z Lu, Y Zhao, H Ma, X Chen, H Jia, H Zheng, G Yang, D Liu, M Tepel, Z Zhu
    JCI Insight, 2022-03-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Cognitive enhancement and neuroprotective effects of OABL, a sesquiterpene lactone in 5xFAD Alzheimer's disease mice model
    Authors: JJ Tang, LF Huang, JL Deng, YM Wang, C Guo, XN Peng, Z Liu, JM Gao
    Redox Biology, 2022-01-08;50(0):102229.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
  21. Development of a Low-Molecular-Weight Abeta42 Detection System Using a Enzyme-Linked Peptide Assay
    Authors: SH Kim, EH Lee, HJ Kim, AR Kim, YE Kim, JH Lee, MY Yoon, SH Koh
    Biomolecules, 2021-12-02;11(12):.
    Species: Human
    Sample Types: CSF
  22. CCR5 antagonist reduces HIV-induced amyloidogenesis, tau pathology, neurodegeneration, and blood-brain barrier alterations in HIV-infected hu-PBL-NSG mice
    Authors: B Bhargavan, SM Woollard, JE McMillan, GD Kanmogne
    Molecular Neurodegeneration, 2021-11-22;16(1):78.
    Species: Human
    Sample Types: Cell Lysates
  23. Microglial Calhm2 regulates neuroinflammation and contributes to Alzheimer's disease pathology
    Authors: J Cheng, Y Dong, J Ma, R Pan, Y Liao, X Kong, X Li, S Li, P Chen, L Wang, Y Yu, Z Yuan
    Science Advances, 2021-08-25;7(35):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Regulation of beta-amyloid production in neurons by astrocyte-derived cholesterol
    Authors: H Wang, JA Kulas, C Wang, DM Holtzman, HA Ferris, SB Hansen
    Proceedings of the National Academy of Sciences of the United States of America, 2021-08-17;118(33):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. Thymosin &beta4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-&kappaB signaling axis in APP/PS1 mice
    Authors: M Wang, LR Feng, ZL Li, KG Ma, KW Chang, XL Chen, PB Yang, SF Ji, YB Ma, H Han, JB Ruganzua, WN Yang, YH Qian
    Journal of Neuroinflammation, 2021-06-28;18(1):146.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Serum Amyloid Beta42 Is Not Eliminated by the Cirrhotic Liver: A Pilot Study
    Authors: R Wiest, TS Weiss, L Danielyan, C Buechler
    Journal of Clinical Medicine, 2021-06-17;10(12):.
    Species: Human
    Sample Types: Serum
  27. CK1delta-Derived Peptides as Novel Tools Inhibiting the Interactions between CK1delta and APP695 to Modulate the Pathogenic Metabolism of APP
    Authors: A Roth, F Gärtner, K Mayer, J Beyrle, I König, U Knippschil, J Bischof
    International Journal of Molecular Sciences, 2021-06-15;22(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer's disease
    Authors: N Brookhouse, S Raman, C Frisch, G Srinivasan, DA Brafman
    Molecular Psychiatry, 2021-04-09;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Amyloid Beta Peptide (A&beta1-42) Reverses the Cholinergic Control of Monocytic IL-1&beta Release
    Authors: K Richter, R Ogiemwonyi, S Wilker, AI Chaveiro, A Agné, M Hecker, M Reichert, AL Amati, KD Schlüter, I Manzini, G Schmalzing, JM McIntosh, W Padberg, V Grau, A Hecker
    J Clin Med, 2020-09-07;9(9):.
    Species: Human
    Sample Types: Plasma
  30. The "develOpment of metabolic and functional markers of Dementia IN Older people" (ODINO) Study: Rationale, Design and Methods
    Authors: A Picca, D Ronconi, HJ Coelho-Jun, R Calvani, F Marini, A Biancolill, J Gervasoni, A Primiano, C Pais, E Meloni, D Fusco, MR Lo Monaco, R Bernabei, MC Cipriani, E Marzetti, R Liperoti
    J Pers Med, 2020-04-09;10(2):.
    Species: Human
    Sample Types: Serum
  31. Urolithin A attenuates memory impairment and neuroinflammation in APP/PS1 mice
    Authors: Z Gong, J Huang, B Xu, Z Ou, L Zhang, X Lin, X Ye, X Kong, D Long, X Sun, X He, L Xu, Q Li, A Xuan
    J Neuroinflammation, 2019-03-14;16(1):62.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. The neuroprotective effect of deep brain stimulation at nucleus basalis of Meynert in transgenic mice with Alzheimer's disease
    Authors: C Huang, H Chu, Y Ma, Z Zhou, C Dai, X Huang, L Fang, Q Ao, D Huang
    Brain Stimul, 2018-08-27;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
  33. MPTAC Determines APP Fragmentation via Sensing Sulfur Amino Acid Catabolism
    Authors: T Suganuma, SK Swanson, M Gogol, TJ Garrett, J Conkright-, L Florens, MP Washburn, JL Workman
    Cell Rep, 2018-08-07;24(6):1585-1596.
    Species: Human
    Sample Types: Cell Lysates
  34. The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
    Authors: P Liu, X Chen, H Zhou, L Wang, Z Zhang, X Ren, F Zhu, Y Guo, X Huang, J Liu, PS Spencer, X Yang
    Oxid Med Cell Longev, 2018-07-02;2018(0):2025914.
    Species: Mouse
    Sample Types: Cell Lysates
  35. HDAC3 negatively regulates spatial memory in a mouse model of Alzheimer's disease
    Authors: X Zhu, S Wang, L Yu, J Jin, X Ye, Y Liu, Y Xu
    Aging Cell, 2017-08-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  36. Folic acid supplementation improves cognitive function by reducing the levels of peripheral inflammatory cytokines in elderly Chinese subjects with MCI
    Sci Rep, 2016-11-23;6(0):37486.
    Species: Human
    Sample Types: Serum

FAQs

  1. Which form of A-beta (1-42) - monomer or oligomer - is detectable in Catalog # DAB142 (Human Amyloid beta (aa1-42) Quantikine ELISA kit)?

    • The Human Amyloid beta (aa1-42) Quantikine ELISA kit recognizes both monomers and oligomers of Human Amyloid beta (aa1-42). The standard in the kit is synthetic human Amyloid beta peptide that cannot oligomerize.

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