Human Androgen R/NR3C4 Isoform 2 Antibody

Catalog #: MAB58764 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB58764-100
MAB58764-SP

Androgen R/NR3C4 in A549 Human Cell Line.

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Human Androgen R/NR3C4 Isoform 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Androgen R/NR3C4 isoform 2 peptide in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1022602

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Androgen R/NR3C4 isoform 2 synthetic peptide
Accession # P10275

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed A549 human lung carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Androgen R/NR3C4 in A549 Human Cell Line. Androgen R/NR3C4 was detected in immersion fixed A549 human lung carcinoma cell line (positive staining) and PC‑3 human prostate cancer cell line (negative staining) using Mouse Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58764) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Androgen R/NR3C4

The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR. AR (Androgen receptor) is a 99 kDa (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins. Due to a high number of Gln and Pro residues, it runs anomalously at 100‑120 kDa in SDS-PAGE. It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, mascrophages, adipocytes, and prostate epithelium. Human AR is 919 amino acids (aa) in length. It contains three discrete domains: a "modulating" N‑terminus (aa 1‑553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa 554‑635), and a ligand-binding domain (aa 672‑917). AR is highly polymorphic at the N‑terminus, with total Gln and Gly residues differing by seven or more residues among individuals. Multiple potential splice forms exist, including an alternative start site at Met189 and a seven aa substitution for aa 1‑538 that generates a 45 kDa isoform. AR does homodimerize, apparently with multiple isotypes. Over aa 661‑920, human and mouse are identical in aa sequence.

Long Name

Androgen Receptor

Entrez Gene IDs

367 (Human)

Alternate Names

AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy