Human Angiopoietin-1 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Saliva, Human Milk
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 611 | 1217 | 2502 | 619 | 1223 | 2496 |
Standard Deviation | 20.8 | 24.2 | 67 | 42.4 | 71.5 | 113 |
CV% | 3.4 | 2 | 2.7 | 6.8 | 5.8 | 4.5 |
Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 587 | 1179 | 2339 | 620 | 1230 | 2533 |
Standard Deviation | 14 | 28.5 | 76.1 | 39.5 | 67.1 | 141 |
CV% | 2.4 | 2.4 | 3.3 | 6.4 | 5.5 | 5.6 |
Recovery
The recovery of Angiopoietin-1 spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 104 | 97-110 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Angiopoietin-1
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Angiopoietin-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Angiogenic and Microvascular Status Alterations after Endovascular Revascularization of Lower Limb Arteries among Patients with Diabetic Foot Syndrome: A Prospective 12-Month Follow-Up Study
Authors: Schönborn, M;Gregorczyk-Maga, I;Batko, K;Maga, M;Bogucka, K;Gawlik, K;Pawlica-Gosiewska, D;Maga, P;
Journal of clinical medicine
Species: Human
Sample Types: Plasma
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Host biomarkers for early identification of severe imported Plasmodium falciparum malaria
Authors: Balerdi-Sarasola, L;Parolo, C;Fleitas, P;Cruz, A;Subirà, C;Rodríguez-Valero, N;Almuedo-Riera, A;Letona, L;Álvarez-Martínez, MJ;Valls, ME;Vera, I;Mayor, A;Muñoz, J;Camprubí-Ferrer, D;
Travel medicine and infectious disease
Species: Human
Sample Types: Whole Blood
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The Concentration of Pro- and Antiangiogenic Factors in Saliva and Gingival Crevicular Fluid Compared to Plasma in Patients with Peripheral Artery Disease and Type 2 Diabetes
Authors: Gregorczyk-Maga, I;Szustkiewicz-Karo?, A;Gajda, M;Kapusta, M;Maga, W;Schönborn, M;
Biomedicines
Species: Human
Sample Types: Plasma
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Circulating Angiogenic Factors and Ischemic Diabetic Foot Syndrome Advancement-A Pilot Study
Authors: Schönborn, M;Gregorczyk-Maga, I;Batko, K;Bogucka, K;Maga, M;P?otek, A;Pasieka, P;S?owi?ska-Solnica, K;Maga, P;
Biomedicines
Species: Human
Sample Types: Plasma
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Increased Angiopoietin-1 and -2 levels in human vitreous are associated with proliferative diabetic retinopathy
Authors: T Tsai, M Alwees, MA Asaad, J Theile, V Kakkassery, HB Dick, T Schultz, SC Joachim
PLoS ONE, 2023-01-20;18(1):e0280488.
Species: Human
Sample Types: Vitreous Humor
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Serum angiopoietin-1 concentration does not distinguish patients with ischaemic stroke from those presenting to hospital with ischaemic stroke mimics
Authors: JV Moxon, AK Kraeuter, J Phie, S Juliano, G Anderson, G Standley, C Sealey, RP White, J Golledge
BMC cardiovascular disorders, 2022-11-04;22(1):462.
Species: Human
Sample Types: Serum
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Upregulation of the oestrogen target gene SIX1 is associated with higher growth speed and decreased survival in HCV-positive women with hepatocellular carcinoma
Authors: RM Critelli, F Milosa, A Romanzi, S Lasagni, G Marcelli, L Di Marco, A Pivetti, F Schepis, D Romagnoli, S Mancarella, F Dituri, ML Martinez-C, G Giannelli, E Villa
Oncology Letters, 2022-09-21;24(5):395.
Species: Human
Sample Types: Serum
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Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients
Authors: M Hultström, K Fromell, A Larsson, B Persson, B Nilsson, SE Quaggin, C Betsholtz, R Frithiof, M Lipcsey, M Jeansson
Biomedicines, 2022-06-06;10(6):.
Species: Human
Sample Types: Plasma
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Platelet-derived growth factor BB is reduced in endometrial endothelial cells of women with abnormal uterine bleeding-endometrial disorder
Authors: S Biswas Shi, Q Lu, D Sun, H Hou, JN Bulmer, BA Innes, DK Hapangama, GE Lash
Oncogene, 2022-02-13;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells
Authors: L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet
Leukemia, 2021-09-23;0(0):.
Species: Human
Sample Types: Plasma
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Mucopolysaccharide polysulfate promotes microvascular stabilization and barrier integrity of dermal microvascular endothelial cells via activation of the angiopoietin-1/Tie2 pathway
Authors: S Fujiwara-S, Y Ueda, M Fujikawa, M Osaki, N Yamanaka, T Matsumoto
Journal of dermatological science, 2021-06-02;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Defined MSC exosome with high yield and purity to improve regenerative activity
Authors: JY Kim, WK Rhim, YI Yoo, DS Kim, KW Ko, Y Heo, CG Park, DK Han
Journal of tissue engineering, 2021-04-20;12(0):2041731421100.
Species: Human
Sample Types: Cell Lysates
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Interleukin-6 as an enhancer of anti-angiogenic therapy for ovarian clear cell carcinoma
Authors: T Seki, N Yanaihara, JS Shapiro, M Saito, J Tabata, R Yokomizo, D Noguchi, T Kuroda, A Kawabata, J Suzuki, K Takahashi, H Matsuzawa, M Miyake, M Takenaka, Y Iida, S Yanagida, A Okamoto
Scientific Reports, 2021-04-08;11(1):7689.
Species: Human
Sample Types: Cell Culture Supernates
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Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells
Authors: AA Hastreiter, GG Dos Santos, EN Makiyama, EWC Santos, P Borelli, RA Fock
The Journal of nutritional biochemistry, 2021-03-08;0(0):108626.
Species: Mouse
Sample Types: Cell Culture Supernates
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Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p
Authors: C Gao, Z Gong, D Wang, J Huang, Y Qian, M Nie, W Jiang, X Liu, H Luo, J Yuan, T Xiang, S An, W Quan, H Wei, J Zhang, R Jiang
Aging (Albany NY), 2019-12-16;11(24):12147-12164.
Species: Human
Sample Types: Serum
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MicroRNA-411 and Its 5'-IsomiR Have Distinct Targets and Functions and Are Differentially Regulated in the Vasculature under Ischemia
Authors: RVCT van der Kw, T Woudenberg, PHA Quax, AY Nossent
Mol. Ther., 2019-10-07;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Systemic Inflammation Precedes Microalbuminuria in Diabetes
Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
Kidney Int Rep, 2019-06-21;4(10):1373-1386.
Species: Human
Sample Types: Serum
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Microcirculatory perfusion disturbances following cardiac surgery with cardiopulmonary bypass are associated with in vitro endothelial hyperpermeability and increased angiopoietin-2 levels
Authors: NAM Dekker, ALI van Leeuwe, WWJ van Strien, J Majolée, R Szulcek, ABA Vonk, PL Hordijk, C Boer, CE van den Br
Crit Care, 2019-04-11;23(1):117.
Species: Human
Sample Types: Plasma
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Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
Authors: M Varas-Godo, S Acuña-Gall, S Venegas-Du, C Hill, A Caceres-Ve, O Realini, LJ Monteiro, G Zavala, M Khoury, R Romero, G Rice, SE Illanes
Stem Cells Int, 2019-01-27;2019(0):1916542.
Species: Human
Sample Types: Cell Culture Supernates
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Different patterns of inflammatory and angiogenic factors are associated with peritoneal small solute transport and peritoneal protein clearance in peritoneal dialysis patients
Authors: Y Shi, H Yan, J Yuan, H Zhang, J Huang, Z Ni, J Qian, W Fang
BMC Nephrol, 2018-05-23;19(1):119.
Species: Human
Sample Types: Serum
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Tie2 as a novel key factor of microangiopathy in systemic sclerosis
Authors: F Moritz, J Schniering, JHW Distler, RE Gay, S Gay, O Distler, B Maurer
Arthritis Res. Ther., 2017-05-25;19(1):105.
Species: Human
Sample Types: Serum
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Alteration Analysis of Bone Marrow Mesenchymal Stromal Cells from De Novo Acute Myeloid Leukemia Patients at Diagnosis
Authors: L Desbourdes, J Javary, T Charbonnie, N Ishac, J Bourgeais, A Iltis, JC Chomel, A Turhan, F Guilloton, K Tarte, MV Demattei, E Ducrocq, F Rouleux-Bo, E Gyan, O Hérault, J Domenech
Stem Cells Dev., 2017-04-10;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
Lab. Invest., 2017-04-10;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Differential regulation of blood flow induced neovascularisation and mural cell recruitment by VEGF and angiopoietin signalling
Authors: David O Bates
J. Physiol. (Lond.), 2017-02-02;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
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Angiopoietin-2, Angiopoietin-1 and subclinical cardiovascular disease in Chronic Kidney Disease
Sci Rep, 2016-12-19;6(0):39400.
Species: Human
Sample Types: Serum
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Circulating Angiopoietin-1 Is Not a Biomarker of Disease Severity or Prognosis in Pulmonary Hypertension
PLoS ONE, 2016-11-01;11(11):e0165982.
Species: Human
Sample Types: Plasma
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Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
Sci Rep, 2016-07-04;6(0):29029.
Species: Human
Sample Types: Cell Culture Supernates
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TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
Sci Adv, 2016-04-01;2(4):e1501290.
Species: Human
Sample Types: Cell Lysates
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Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
PLoS ONE, 2015-08-26;10(8):e0136205.
Species: Human
Sample Types: Plasma
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EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
Authors: Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S
Stem Cells, 2015-06-23;33(9):2838-49.
Species: Human
Sample Types: Cell Culture Supernates
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Angiopoietin-2 and angiopoietin-2/angiopoietin-1 ratio as indicators of potential severity of Plasmodium vivax malaria in patients with thrombocytopenia.
Authors: Gomes L, Alves-Junior E, Rodrigues-Jesus C, Nery A, Gasquez-Martin T, Fontes C
PLoS ONE, 2014-10-02;9(10):e109246.
Species: Human
Sample Types: Serum
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Serum angiopoietin-2 and soluble VEGFR-2 levels predict malignancy of ovarian neoplasm and poor prognosis in epithelial ovarian cancer.
Authors: Sallinen H, Heikura T, Koponen J, Kosma V, Heinonen S, Yla-Herttuala S, Anttila M
BMC Cancer, 2014-09-23;14(0):696.
Species: Human
Sample Types: Serum
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Impaired function of the Tie-2 receptor contributes to vascular leakage and lethality in anthrax.
Proc Natl Acad Sci U S A, 2012-06-04;109(25):10024-9.
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Serum
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Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
BMC Cancer, 2011-07-05;11(0):286.
Species: Human
Sample Types: Serum
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Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
Diabetologia, 2011-06-10;54(9):2358-67.
Species: Human
Sample Types: Serum
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CD34+ progenitor cells likely are involved in the good functional recovery after intracerebral hemorrhage in humans.
Authors: Sobrino T, Arias S, Perez-Mato M, Agulla J, Brea D, Rodriguez-Yanez M, Castillo J
J. Neurosci. Res., 2011-04-12;89(7):979-85.
Species: Human
Sample Types: Serum
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Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
Crit. Care Med., 2011-04-01;0(0):.
Species: Human
Sample Types: Plasma
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Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
Authors: Boucherat O, Franco-Montoya ML, Delacourt C, Martinovic J, Masse V, Elie C, Thebaud B, Benachi A, Bourbon JR
Am. J. Physiol. Lung Cell Mol. Physiol., 2010-03-26;298(6):L849-56.
Species: Human
Sample Types: Tissue Homogenates
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Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
Exp. Hematol., 2009-06-01;37(6):673-8.
Species: Human
Sample Types: Plasma
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Colorectal resection is associated with persistent proangiogenic plasma protein changes: postoperative plasma stimulates in vitro endothelial cell growth, migration, and invasion.
Authors: Shantha Kumara HM, Feingold D, Kalady M, Dujovny N, Senagore A, Hyman N, Cekic V, Whelan RL
Ann. Surg., 2009-06-01;249(6):973-7.
Species: Human
Sample Types: Plasma
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Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation.
Authors: Hurliman AK, Speroff L, Stouffer RL, Patton PE, Lee A, Molskness TA
Fertil. Steril., 2009-05-23;93(5):1493-9.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Serum
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Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors.
Authors: Serefhanoglu S, Goker H, Buyukasik Y, Turgut M, Sayinalp N, Haznedaroglu IC, Aksu S, Akman U, Ozcebe OI
Ann. Hematol., 2008-12-11;88(7):667-71.
Species: Human
Sample Types: Plasma
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Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
Exp. Hematol., 2008-05-27;36(8):1035-46.
Species: Human
Sample Types: Cell Culture Supernates
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Structural stability of neoangiogenic intramyocardial microvessels supports functional recovery in chronic ischemic myocardium.
Authors: Shim WS, Lim SY, Li SQ, Gu Y, Ong HC, Song IC, Chuah SC, Wong P
J. Mol. Cell. Cardiol., 2008-04-27;45(1):70-80.
Species: Porcine
Sample Types: Tissue Homogenates
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Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
Ann. Surg., 2008-02-01;247(2):320-6.
Species: Human
Sample Types: Plasma
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Relationship of soluble CD40 ligand to vascular endothelial growth factor, angiopoietins, and tissue factor in atrial fibrillation: a link among platelet activation, angiogenesis, and thrombosis?
Authors: Choudhury A, Freestone B, Patel J, Lip GY
Chest, 2007-12-01;132(6):1913-9.
Species: Human
Sample Types: Plasma
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Angiopoietin-2 as a contributing factor of exercise-induced bronchoconstriction in asthmatic patients receiving inhaled corticosteroid therapy.
Authors: Kanazawa H, Tochino Y, Asai K
J. Allergy Clin. Immunol., 2007-11-26;121(2):390-5.
Species: Human
Sample Types: Sputum
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Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia.
Authors: Lee CY, Tien HF, Hu CY, Chou WC, Lin LI
Br. J. Cancer, 2007-09-11;97(7):877-82.
Species: Human
Sample Types: Plasma
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Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
Br. J. Haematol., 2007-06-01;137(6):560-8.
Species: Human
Sample Types: Serum
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Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
Stem Cells, 2007-04-19;25(8):1862-71.
Species: Human
Sample Types: Cell Culture Supernates
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Roles of angiopoietin-1 and angiopoietin-2 on airway microvascular permeability in asthmatic patients.
Authors: Kanazawa H, Nomura S, Asai K
Chest, 2007-04-01;131(4):1035-41.
Species: Human
Sample Types: Sputum
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Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers.
Authors: Niedzwiecki S, Stepien T, Kopec K, Kuzdak K, Komorowski J, Krupinski R, Stepien H
Cytokine, 2007-03-19;36(5):291-5.
Species: Human
Sample Types: Serum
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Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
Authors: Caine GJ, Ryan P, Lip GY, Blann AD
Cancer Lett., 2007-01-19;251(2):296-301.
Species: Human
Sample Types: Plasma
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Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
Cancer Lett., 2006-08-07;248(1):131-6.
Species: Human
Sample Types: Plasma
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