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Human Angiopoietin-2 ELISA Kit - Quantikine

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Human Angiopoietin-2 ELISA Standard Curve
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Summary Sample Values Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Angiopoietin-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL)
Sensitivity
21.3 pg/mL
Assay Range
46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human Angiopoietin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human Angiopoietin-2 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum (n=60)24941065-89071341
EDTA plasma (n=35)19641071-4389808
Heparin plasma (n=35)20491009-4973913

Saliva - Nine samples were evaluated for the presence of human Angiopoietin-2 in this assay and ranged from 247-822 pg/mL.

Cell Culture Supernates - Two cultures of HUVEC human umbilical vein endothelial cells were grown to confluency in EGM supplemented with 2% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate and bovine brain extract. Aliquots of the cell culture supernates were removed and assayed for levels of human Angiopoietin-2.

SampleValues (pg/mL)
HUVEC Culture 12326
HUVEC Culture 27611

Note: Significant levels of Angiopoietin-2 are found in fetal bovine, bovine, porcine, equine, and rabbit serum. The background level of Angiopoietin-2 in control medium should be determined and subtracted from samples of conditioned medium.





Product Summary

The Quantikine Human Angiopoietin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-2 in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human Angiopoietin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-2.

Recovery

The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-113
EDTA Plasma (n=4) 95 85-104
Heparin Plasma (n=4) 94 87-104
Serum (n=4) 100 90-107

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Angiopoietin-2 ELISA Linearity

Scientific Data

N/A Human Angiopoietin-2 ELISA Standard Curve View Larger

Human Angiopoietin-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names:
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Angiopoietin-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

103 Citations: Showing 1 - 10
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  1. Fluid bolus increases plasma hyaluronan concentration compared to follow-up strategy without a bolus in oliguric intensive care unit patients
    Authors: Serlo, M;Inkinen, N;Lakkisto, P;Valkonen, M;Pulkkinen, A;Selander, T;Pettilä, V;Vaara, ST;
    Scientific reports
    Species: Human
    Sample Types: Plasma, Urine
  2. The possible dual role of Ang-2 in the prognosis of pancreatic cancer
    Authors: Roos-Mattila, M;Kaprio, T;Mustonen, H;Hagström, J;Saharinen, P;Haglund, C;Seppänen, H;
    Scientific reports
    Species: Human
    Sample Types: Serum
  3. Endothelial CD34 expression and regulation of immune cell response in-vitro
    Authors: Arakelian, L;Lion, J;Churlaud, G;Bargui, R;Thierry, B;Mutabazi, E;Bruneval, P;Alberdi, AJ;Doliger, C;Veyssiere, M;Larghero, J;Mooney, N;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Host biomarkers for early identification of severe imported Plasmodium falciparum malaria
    Authors: Balerdi-Sarasola, L;Parolo, C;Fleitas, P;Cruz, A;Subirà, C;Rodríguez-Valero, N;Almuedo-Riera, A;Letona, L;Álvarez-Martínez, MJ;Valls, ME;Vera, I;Mayor, A;Muñoz, J;Camprubí-Ferrer, D;
    Travel medicine and infectious disease
    Species: Human
    Sample Types: Whole Blood
  5. High Plasma Angiopoietin-2 Levels Predict the Need to Initiate Dialysis within Two Years in Patients with Chronic Kidney Disease
    Authors: Szymczak, A;Kusztal, M;Go??biowski, T;Letachowicz, K;Go?dzik, A;Ko?cielska-Kasprzak, K;Tukiendorf, A;Krajewska, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  6. Adipose tissue specific CCL18 associates with cardiometabolic diseases in non-obese individuals implicating CD4+ T cells
    Authors: N Subramania, K Hofwimmer, B Tavira, L Massier, DP Andersson, P Arner, J Laurenciki
    Cardiovascular Diabetology, 2023-04-12;22(1):84.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Increased Angiopoietin-1 and -2 levels in human vitreous are associated with proliferative diabetic retinopathy
    Authors: T Tsai, M Alwees, MA Asaad, J Theile, V Kakkassery, HB Dick, T Schultz, SC Joachim
    PLoS ONE, 2023-01-20;18(1):e0280488.
    Species: Human
    Sample Types: Vitreous Humor
  8. Acute Changes in Serum Creatinine and Kinetic Glomerular Filtration Rate Estimation in Early Phase of Acute Pancreatitis
    Authors: P Dumnicka, M Mazur-Lask, P Ceranowicz, M Sporek, W Kolber, J Tiso?czyk, M Ku?niewski, B Maziarz, B Ku?nierz-C
    Journal of Clinical Medicine, 2022-10-19;11(20):.
    Species: Human
    Sample Types: Serum
  9. Upregulation of the oestrogen target gene SIX1 is associated with higher growth speed and decreased survival in HCV-positive women with hepatocellular carcinoma
    Authors: RM Critelli, F Milosa, A Romanzi, S Lasagni, G Marcelli, L Di Marco, A Pivetti, F Schepis, D Romagnoli, S Mancarella, F Dituri, ML Martinez-C, G Giannelli, E Villa
    Oncology Letters, 2022-09-21;24(5):395.
    Species: Human
    Sample Types: Serum
  10. Selective adipocyte loss of Angiopoietin-2 prompts female-specific obesity and metabolic syndrome
    Authors: B Ni, S Chen, KA Ryan, ML Maitland, JS Farrar, M Witzenrath, B Gubier, C Serdjebi, K Bertotti, R Wang, FN Salloum, L Marino, BD Mitchell, FS Celi
    Molecular Metabolism, 2022-08-30;0(0):101588.
    Species: Human
    Sample Types: Serum
  11. ANGPTL4 influences the therapeutic response of neovascular age-related macular degeneration patients by promoting choroidal neovascularization
    Authors: Y Qin, A Dinabandhu, X Cao, JC Sanchez, K Jee, M Rodrigues, C Guo, J Zhang, J Vancel, D Menon, NS Khan, T Ma, SY Tzeng, YJ Daoud, JJ Green, GL Semenza, S Montaner, A Sodhi
    JCI Insight, 2022-07-08;0(0):.
    Species: Human
    Sample Types: Aqueous Humor
  12. Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients
    Authors: M Hultström, K Fromell, A Larsson, B Persson, B Nilsson, SE Quaggin, C Betsholtz, R Frithiof, M Lipcsey, M Jeansson
    Biomedicines, 2022-06-06;10(6):.
    Species: Human, Mouse
    Sample Types: Plasma
  13. Cholecalciferol supplementation and angiogenic markers in chronic kidney disease
    Authors: J Kaur, K Kamboj, AK Yadav, P Kaur, V Kumar, V Jha
    PLoS ONE, 2022-06-03;17(6):e0268946.
    Species: Human
    Sample Types: Serum
  14. The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis
    Authors: Y Yamada, Y Zhong, S Miki, A Taura, TH Rabbitts
    Scientific Reports, 2022-05-04;12(1):7226.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Endothelial dysfunction and altered endothelial biomarkers in patients with post-COVID-19 syndrome and chronic fatigue syndrome (ME/CFS)
    Authors: M Haffke, H Freitag, G Rudolf, M Seifert, W Doehner, N Scherbakov, L Hanitsch, K Wittke, S Bauer, F Konietschk, F Paul, J Bellmann-S, C Kedor, C Scheibenbo, F Sotzny
    Journal of Translational Medicine, 2022-03-22;20(1):138.
    Species: Human
    Sample Types: Serum
  16. Angpt2/Tie2 autostimulatory loop controls tumorigenesis
    Authors: NM Karabid, T Wiedemann, S Gulde, H Mohr, RC Segaran, J Geppert, M Rohm, G Vitale, G Gaudenzi, A Dicitore, DP Ankerst, Y Chen, R Braren, G Kaissis, F Schilling, M Schillmaie, G Eisenhofer, S Herzig, F Roncaroli, JB Honegger, NS Pellegata
    Embo Molecular Medicine, 2022-03-10;0(0):e14364.
    Species: Human
    Sample Types: Plasma
  17. Elevated plasma levels of epithelial and endothelial cell markers in COVID-19 survivors with reduced lung diffusing capacity six months after hospital discharge
    Authors: O Sibila, L Perea, N Albacar, J Moisés, T Cruz, N Mendoza, B Solarat, G Lledó, G Espinosa, JA Barberà, JR Badia, A Agustí, J Sellarés, R Faner
    Respiratory Research, 2022-02-21;23(1):37.
    Species: Human
    Sample Types: Plasma
  18. Prognostic Value of Tie2-Expressing Monocytes in Chronic Lymphocytic Leukemia Patients
    Authors: J Wo?, S Chocholska, W Kowalska, W Tomczak, A Szyma?ska, A Karczmarcz, A Szuster-Ci, A Wojciechow, A Bojarska-J
    Cancers, 2021-06-05;13(11):.
    Species: Human
    Sample Types: Plasma
  19. Mucopolysaccharide polysulfate promotes microvascular stabilization and barrier integrity of dermal microvascular endothelial cells via activation of the angiopoietin-1/Tie2 pathway
    Authors: S Fujiwara-S, Y Ueda, M Fujikawa, M Osaki, N Yamanaka, T Matsumoto
    Journal of dermatological science, 2021-06-02;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Endothelial glycocalyx degradation and disease severity in Plasmodium vivax and Plasmodium knowlesi malaria
    Authors: BE Barber, MJ Grigg, KA Piera, Y Chen, T William, JB Weinberg, TW Yeo, NM Anstey
    Scientific Reports, 2021-05-07;11(1):9741.
    Species: Human
    Sample Types: Plasma
  21. Interleukin-6 as an enhancer of anti-angiogenic therapy for ovarian clear cell carcinoma
    Authors: T Seki, N Yanaihara, JS Shapiro, M Saito, J Tabata, R Yokomizo, D Noguchi, T Kuroda, A Kawabata, J Suzuki, K Takahashi, H Matsuzawa, M Miyake, M Takenaka, Y Iida, S Yanagida, A Okamoto
    Scientific Reports, 2021-04-08;11(1):7689.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Influence of serum inflammatory cytokines on cytochrome P450 drug metabolising activity during breast cancer chemotherapy: a patient feasibility study
    Authors: RLI Crake, MR Strother, E Phillips, MP Doogue, M Zhang, CMA Frampton, BA Robinson, MJ Currie
    Scientific Reports, 2021-03-11;11(1):5648.
    Species: Human
    Sample Types: Serum
  23. Blood angiopoietin-2 predicts liver angiogenesis and fibrosis in hepatitis C patients
    Authors: Y Osawa, S Yoshio, Y Aoki, M Korenaga, M Imamura, T Oide, M Okawara, H Kawai, Y Tsutsui, Y Yoshida, S Yoshikawa, T Mori, T Yamazoe, T Kanto
    BMC gastroenterology, 2021-02-08;21(1):55.
    Species: Human
    Sample Types: Plasma
  24. Vascular density with optical coherence tomography angiography and systemic biomarkers in low and high cardiovascular risk patients
    Authors: MA Hannappe, L Arnould, A Méloux, B Mouhat, F Bichat, M Zeller, Y Cottin, C Binquet, C Vergely, C Creuzot-Ga, C Guenancia
    Sci Rep, 2020-10-07;10(1):16718.
    Species: Human
    Sample Types: Serum
  25. Combining 1,4-dihydroxy quininib with Bevacizumab/FOLFOX alters angiogenic and inflammatory secretions in ex vivo colorectal tumors
    Authors: SA Kennedy, ME Morrissey, MR Dunne, F O'Connell, CT Butler, MC Cathcart, AM Buckley, BJ Mehigan, JO Larkin, P McCormick, BN Kennedy, J O'Sullivan
    BMC Cancer, 2020-10-02;20(1):952.
    Species: Human
    Sample Types: Tissue Culture Supernates
  26. Clinical value of selected markers of angiogenesis, inflammation, insulin resistance and obesity in type 1 endometrial cancer
    Authors: KM Terlikowsk, B Dobrzycka, R Terlikowsk, A Sienkiewic, M Kinalski, SJ Terlikowsk
    BMC Cancer, 2020-09-25;20(1):921.
    Species: Human
    Sample Types: Serum
  27. Does Beta-Trace Protein (BTP) Outperform Cystatin C as a Diagnostic Marker of Acute Kidney Injury Complicating the Early Phase of Acute Pancreatitis?
    Authors: J Wajda, P Dumnicka, M Sporek, B Maziarz, W Kolber, A Z?bek-Adam, P Ceranowicz, M Ku?niewski, B Ku?nierz-C
    J Clin Med, 2020-01-11;9(1):.
    Species: Human
    Sample Types: Serum
  28. Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p
    Authors: C Gao, Z Gong, D Wang, J Huang, Y Qian, M Nie, W Jiang, X Liu, H Luo, J Yuan, T Xiang, S An, W Quan, H Wei, J Zhang, R Jiang
    Aging (Albany NY), 2019-12-16;11(24):12147-12164.
    Species: Human
    Sample Types: Serum
  29. Adhesion of T cells to endothelial cells facilitates blinatumomab-associated neurologic adverse events
    Authors: M Klinger, G Zugmaier, V Nägele, ME Goebeler, C Brandl, M Stelljes, H Lassmann, A von Stacke, RC Bargou, P Kufer
    Cancer Res., 2019-10-29;0(0):.
    Species: Human
    Sample Types: Plasma
  30. Relationship of mottling score, skin microcirculatory perfusion indices and biomarkers of endothelial dysfunction in patients with septic shock: an observational study
    Authors: S Kazune, A Caica, K Volceka, O Suba, U Rubins, A Grabovskis
    Crit Care, 2019-09-11;23(1):311.
    Species: Human
    Sample Types: Plasma
  31. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
    Kidney Int Rep, 2019-06-21;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  32. Microcirculatory perfusion disturbances following cardiac surgery with cardiopulmonary bypass are associated with in vitro endothelial hyperpermeability and increased angiopoietin-2 levels
    Authors: NAM Dekker, ALI van Leeuwe, WWJ van Strien, J Majolée, R Szulcek, ABA Vonk, PL Hordijk, C Boer, CE van den Br
    Crit Care, 2019-04-11;23(1):117.
    Species: Human
    Sample Types: Plasma
  33. Fibroblast growth factor 23, endothelium biomarkers and acute kidney injury in critically-ill patients
    Authors: FM de Oliveir, CB Araújo, DF de Freitas, BFT Arruda, LJM de Macêdo, VB Salles, GC Meneses, AMC Martins, AB Libório
    J Transl Med, 2019-04-11;17(1):121.
    Species: Human
    Sample Types: Plasma
  34. Distribution and Activity of Lenvatinib in Brain Tumor Models of Human Anaplastic Thyroid Cancer Cells in Severe Combined Immune Deficient Mice
    Authors: R Wang, T Yamada, S Arai, K Fukuda, H Taniguchi, A Tanimoto, A Nishiyama, S Takeuchi, K Yamashita, K Ohtsubo, J Matsui, N Onoda, E Hirata, S Taira, S Yano
    Mol. Cancer Ther., 2019-03-29;18(5):947-956.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia
    Authors: M Varas-Godo, S Acuña-Gall, S Venegas-Du, C Hill, A Caceres-Ve, O Realini, LJ Monteiro, G Zavala, M Khoury, R Romero, G Rice, SE Illanes
    Stem Cells Int, 2019-01-27;2019(0):1916542.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Does the Automatic Measurement of Interleukin 6 Allow for Prediction of Complications during the First 48 h of Acute Pancreatitis?
    Authors: W Kolber, P Dumnicka, M Maraj, B Ku?nierz-C, P Ceranowicz, M P?dziwiatr, B Maziarz, M Mazur-Lask, M Ku?niewski, M Sporek, J Walocha
    Int J Mol Sci, 2018-06-20;19(6):.
    Species: Human
    Sample Types: Serum
  37. Different patterns of inflammatory and angiogenic factors are associated with peritoneal small solute transport and peritoneal protein clearance in peritoneal dialysis patients
    Authors: Y Shi, H Yan, J Yuan, H Zhang, J Huang, Z Ni, J Qian, W Fang
    BMC Nephrol, 2018-05-23;19(1):119.
    Species: Human
    Sample Types: Serum
  38. Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis
    Authors: C Robinson, L Tsang, A Solomon, AJ Woodiwiss, S Gunter, M Mer, HC Hsu, M Gomes, GR Norton, AME Millen, PH Dessein
    Peptides, 2018-02-21;102(0):31-37.
    Species: Human
    Sample Types: Plasma
  39. Myeloperoxidase-derived 2-chlorofatty acids contribute to human sepsis mortality via acute respiratory distress syndrome
    Authors: NJ Meyer, JP Reilly, R Feng, JD Christie, SL Hazen, CJ Albert, JD Franke, CL Hartman, J McHowat, DA Ford
    JCI Insight, 2017-12-07;2(23):.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Tie2 as a novel key factor of microangiopathy in systemic sclerosis
    Authors: F Moritz, J Schniering, JHW Distler, RE Gay, S Gay, O Distler, B Maurer
    Arthritis Res. Ther., 2017-05-25;19(1):105.
    Species: Human
    Sample Types: Serum
  41. Angiopoietin-2 and soluble Tie-2 receptor plasma levels in children with obstructive sleep apnea and obesity
    Authors: D Gozal, A Khalyfa, Z Qiao, DL Smith, MF Philby, D Koren, L Kheirandis
    Obesity (Silver Spring), 2017-05-05;0(0):.
    Species: Human
    Sample Types: Plasma
  42. Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D
    Authors: S Demolli, A Doddaballa, K Devraj, K Stark, Y Manavski, A Eckart, CM Zehendner, T Lucas, T Korff, M Hecker, S Massberg, S Liebner, D Kaluza, RA Boon, S Dimmeler
    Cardiovasc. Res., 2017-05-01;113(6):681-691.
    Species: Human
    Sample Types: Cell Lysates
  43. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
    Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
    Lab. Invest., 2017-04-10;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Serum Concentrations of Angiopoietin-2 and Soluble fms-Like Tyrosine Kinase 1 (sFlt-1) Are Associated with Coagulopathy among Patients with Acute Pancreatitis
    Authors: P Dumnicka, B Ku?nierz-C, M Sporek, M Mazur-Lask, K Gil, M Ku?niewski, P Ceranowicz, Z Warzecha, A Dembi?ski, J Bonior, R Dro?d?
    Int J Mol Sci, 2017-04-02;18(4):.
    Species: Human
    Sample Types: Serum
  45. Profiling of selected angiogenesis-related genes in serous ovarian cancer patients
    Authors: P Ku?, R Charkiewic, D Klasa-Mazu, T Milczek, B Mroczko, J Nikli?ski, P Lauda?ski
    Adv Med Sci, 2017-02-21;62(1):116-120.
    Species: Human
    Sample Types: Cell Lysates
  46. Angiopoietin-2, Angiopoietin-1 and subclinical cardiovascular disease in Chronic Kidney Disease
    Sci Rep, 2016-12-19;6(0):39400.
    Species: Human
    Sample Types: Serum
  47. IL-35 Inhibits Angiogenesis through VEGF/Ang2/Tie2 Pathway in Rheumatoid Arthritis
    Authors: Shenyi Jiang
    Cell. Physiol. Biochem, 2016-12-14;40(5):1105-1116.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016-07-04;6(0):29029.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
    Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
    J Am Heart Assoc, 2016-04-18;5(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
    Sci Adv, 2016-04-01;2(4):e1501290.
    Species: Human
    Sample Types: Cell Lysates
  51. Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
    Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
    PLoS ONE, 2015-08-26;10(8):e0136205.
    Species: Human
    Sample Types: Plasma
  52. Kidney function, endothelial activation and atherosclerosis in black and white Africans with rheumatoid arthritis.
    Authors: Dessein, Patrick, Hsu, Hon-Chun, Tsang, Linda, Millen, Aletta M, Woodiwiss, Angela J, Norton, Gavin R, Solomon, Ahmed, Gonzalez-Gay, Miguel A
    PLoS ONE, 2015-03-25;10(3):e0121693.
    Species: Human
    Sample Types: Plasma
  53. Combining bevacizumab and chemoradiation in rectal cancer. Translational results of the AXEBeam trial.
    Authors: Verstraete M, Debucquoy A, Dekervel J, Van Pelt J, Verslype C, Devos E, Chiritescu G, Dumon K, D'Hoore A, Gevaert O, Sagaert X, Van Cutsem E, Haustermans K
    Br J Cancer, 2015-03-17;112(8):1314-25.
    Species: Human
    Sample Types: Whole Blood
  54. A randomized phase II study of capecitabine-based chemoradiation with or without bevacizumab in resectable locally advanced rectal cancer: clinical and biological features.
    Authors: Salazar R, Capdevila J, Laquente B, Manzano J, Pericay C, Villacampa M, Lopez C, Losa F, Safont M, Gomez A, Alonso V, Escudero P, Gallego J, Sastre J, Gravalos C, Biondo S, Palacios A, Aranda E
    BMC Cancer, 2015-02-26;15(0):60.
    Species: Human
    Sample Types: Plasma
  55. Mechanism of angiopoietin-1 upregulation in Kaposi's sarcoma-associated herpesvirus-infected PEL cell lines.
    Authors: Zheng X, Ohsaki E, Ueda K
    J Virol, 2015-01-28;89(9):4786-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Association of angiopoietin-2 with renal outcome in chronic kidney disease.
    Authors: Tsai, Yi-Chun, Chiu, Yi-Wen, Tsai, Jer-Chia, Kuo, Hung-Tie, Lee, Su-Chu, Hung, Chi-Chih, Lin, Ming-Yen, Hwang, Shang-Jy, Kuo, Mei-Chua, Chen, Hung-Chu
    PLoS ONE, 2014-10-03;9(10):e108862.
    Species: Human
    Sample Types: Plasma
  57. Angiopoietin-2 and angiopoietin-2/angiopoietin-1 ratio as indicators of potential severity of Plasmodium vivax malaria in patients with thrombocytopenia.
    Authors: Gomes L, Alves-Junior E, Rodrigues-Jesus C, Nery A, Gasquez-Martin T, Fontes C
    PLoS ONE, 2014-10-02;9(10):e109246.
    Species: Human
    Sample Types: Serum
  58. Serum angiopoietin-2 and soluble VEGFR-2 levels predict malignancy of ovarian neoplasm and poor prognosis in epithelial ovarian cancer.
    Authors: Sallinen H, Heikura T, Koponen J, Kosma V, Heinonen S, Yla-Herttuala S, Anttila M
    BMC Cancer, 2014-09-23;14(0):696.
    Species: Human
    Sample Types: Serum
  59. Angiopoietin-2 and biliary diseases: elevated serum, but not bile levels are associated with cholangiocarcinoma.
    Authors: Voigtlander T, David S, Thamm K, Schlue J, Metzger J, Manns M, Lankisch T
    PLoS ONE, 2014-05-13;9(5):e97046.
    Species: Human
    Sample Types: Bile
  60. Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease.
    Authors: Krautkramer E, Grouls S, Hettwer D, Rafat N, Tonshoff B, Zeier M
    J Virol, 2013-10-23;88(1):483-9.
    Species: Human
    Sample Types: Plasma
  61. Clinical outcome, proteome kinetics and angiogenic factors in serum after thermoablation of colorectal liver metastases.
    Authors: Wertenbroek, Marieke, Schepers, Marianne, Kamminga-Rasker, Hannetta, Bottema, Jan T, Muller Kobold, Anneke C, Roelofsen, Han, de Jong, Koert P
    BMC Cancer, 2013-05-30;13(1):266.
    Species: Human
    Sample Types: Serum
  62. Intermediate monocytes but not TIE2-expressing monocytes are a sensitive diagnostic indicator for colorectal cancer.
    Authors: Schauer D, Starlinger P, Reiter C, Jahn N, Zajc P, Buchberger E, Bachleitner-Hofmann T, Bergmann M, Stift A, Gruenberger T, Brostjan C
    PLoS ONE, 2012-09-04;7(9):e44450.
    Species: Human
    Sample Types: Plasma
  63. Impaired function of the Tie-2 receptor contributes to vascular leakage and lethality in anthrax.
    Proc Natl Acad Sci U S A, 2012-06-04;109(25):10024-9.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Serum
  64. Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
    Authors: Llovet JM, Pena CE, Lathia CD, Shan M, Meinhardt G, Bruix J, Pallota MG, Zarba JJ, Boyer M, Riordan S, Strickland A, Tebbutt N, Thomson B, Borbath I, De Greve J, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Barrios C, Cosme de Oliveira A, Kotzev I, Takov D, Tchernev K, Burak K, Ma M, Metrakos P, Olweny C, Sherman M, Gamargo Garate C, Martinez-Castillo J, Beaugrand M, Bennouna J, Blanc J-, Bronowicki J-, Degos F, Dominguez S, Grange J-, Hillon P, Raoul J-, Seitz J-, Blum H, Buggisch P, Caspary W, Dollinger M, Gerken G, Goke B, Gregor M, Greten T, Haussinger D, Hilgard P, Scherübl J, Scheulen M, Schmid R, Spengler U, Wiest R, Zeuzem S, Arvanitakis C, Germanidis G, Katsos I, Figer A, Stemmer S, Amadori D, Bolondi L, Cognetti F, Craxi A, Farinati F, Gridelli C, Martoni A, Mazzaferro V, Porta C, Ricci S, Sangiovanni A, Santoro A, Trevisani F, Cisnero Garza LE, Gane E, O'Donnell A, Leon J, Lozano A, Jassem J, Rydzewska G, Szawlowski A, Tomczak P, Badulescu F, Miron L, Kubyshkin V, Bruix J, Forner A, Bustamante Schneider J, Diago M, Montero Alvarez JL, Pascual S, Ruíz del Arbol L, Sangro B, Solá R, Tabernero J, Muellhaupt B, Roth A, Jeffry Evans TR, Falk S, Meyer T, Reeves H, Ross P, Befeler A, Boyer T, Britten C, Byrne T, Garcia-Tsao G, Gold P, Goldenberg A, Heuman D, Kennedy P, Koch A, Llovet JM, Marrero J, Schilsky M, Schwartz J, Schwartz M
    Clin. Cancer Res., 2012-02-28;18(8):2290-300.
    Species: Human
    Sample Types: Plasma
  65. Circulating angiopoietin-2 and soluble Tie-2 in type 2 diabetes mellitus: a cross-sectional study.
    Authors: Rasul S, Reiter MH, Ilhan A, Lampichler K, Wagner L, Kautzky-Willer A
    Cardiovasc Diabetol, 2011-06-23;10(0):55.
    Species: Human
    Sample Types: Plasma
  66. Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
    Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
    Crit. Care Med., 2011-04-01;0(0):.
    Species: Human
    Sample Types: Plasma
  67. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
    Authors: Volkova E, Willis JA, Wells JE, Robinson BA, Dachs GU, Currie MJ
    Br. J. Cancer, 2010-11-16;104(1):51-9.
    Species: Human
    Sample Types: Serum
  68. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
    Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
    Cardiovasc. Res., 2010-07-29;89(1):157-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. Angiopoietin-2 plasma dosage predicts time to first treatment and overall survival in chronic lymphocytic leukemia.
    Authors: Maffei R, Martinelli S, Santachiara R
    Blood, 2010-04-09;116(4):584-92.
    Species: Human
    Sample Types: Plasma
  70. Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
    Authors: Boucherat O, Franco-Montoya ML, Delacourt C, Martinovic J, Masse V, Elie C, Thebaud B, Benachi A, Bourbon JR
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010-03-26;298(6):L849-56.
    Species: Human
    Sample Types: Tissue Homogenates
  71. Effect of prostaglandin F2 alpha on local luteotropic and angiogenic factors during induced functional luteolysis in the bovine corpus luteum.
    Authors: Berisha B, Meyer HH, Schams D
    Biol. Reprod., 2010-01-07;82(5):940-7.
    Species: Bovine
    Sample Types: Tissue Homogenates
  72. Increased plasma EPO and MIP-1 alpha are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts.
    Authors: Labonte L, Li Y, Yang L, Gillingham A, Halpenny M, Giulivi A, Sills T, Evans K, Zanke B, Allan DS
    Exp. Hematol., 2009-06-01;37(6):673-8.
    Species: Human
    Sample Types: Plasma
  73. Colorectal resection is associated with persistent proangiogenic plasma protein changes: postoperative plasma stimulates in vitro endothelial cell growth, migration, and invasion.
    Authors: Shantha Kumara HM, Feingold D, Kalady M, Dujovny N, Senagore A, Hyman N, Cekic V, Whelan RL
    Ann. Surg., 2009-06-01;249(6):973-7.
    Species: Human
    Sample Types: Plasma
  74. Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation.
    Authors: Hurliman AK, Speroff L, Stouffer RL, Patton PE, Lee A, Molskness TA
    Fertil. Steril., 2009-05-23;93(5):1493-9.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Serum
  75. Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis.
    Authors: Bourlev V, Iljasova N, Adamyan L, Larsson A, Olovsson M
    Fertil. Steril., 2009-03-25;94(1):52-7.
    Species: Human
    Sample Types: Serum
  76. Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma.
    Authors: Helfrich I, Edler L, Sucker A, Thomas M, Christian S, Schadendorf D, Augustin HG
    Clin. Cancer Res., 2009-02-15;15(4):1384-92.
    Species: Human
    Sample Types: Serum
  77. Changes in vascular endothelial growth factor, angiopoietins, and Tie-2 levels with G-CSF stimulation in healthy donors.
    Authors: Serefhanoglu S, Goker H, Buyukasik Y, Turgut M, Sayinalp N, Haznedaroglu IC, Aksu S, Akman U, Ozcebe OI
    Ann. Hematol., 2008-12-11;88(7):667-71.
    Species: Human
    Sample Types: Plasma
  78. Angiopoietin-2 is associated with decreased endothelial nitric oxide and poor clinical outcome in severe falciparum malaria.
    Authors: Yeo TW, Lampah DA, Gitawati R, Tjitra E, Kenangalem E, Piera K, Price RN, Duffull SB, Celermajer DS, Anstey NM
    Proc. Natl. Acad. Sci. U.S.A., 2008-10-28;105(44):17097-102.
    Species: Human
    Sample Types: Plasma
  79. Plasma levels of soluble Tie2 and vascular endothelial growth factor distinguish critical limb ischemia from intermittent claudication in patients with peripheral arterial disease.
    Authors: Findley CM, Mitchell RG, Duscha BD, Annex BH, Kontos CD
    J. Am. Coll. Cardiol., 2008-07-29;52(5):387-93.
    Species: Human
    Sample Types: Plasma
  80. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008-05-27;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Structural stability of neoangiogenic intramyocardial microvessels supports functional recovery in chronic ischemic myocardium.
    Authors: Shim WS, Lim SY, Li SQ, Gu Y, Ong HC, Song IC, Chuah SC, Wong P
    J. Mol. Cell. Cardiol., 2008-04-27;45(1):70-80.
    Species: Porcine
    Sample Types: Tissue Homogenates
  82. Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
    Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
    Ann. Surg., 2008-02-01;247(2):320-6.
    Species: Human
    Sample Types: Plasma
  83. Relationship of soluble CD40 ligand to vascular endothelial growth factor, angiopoietins, and tissue factor in atrial fibrillation: a link among platelet activation, angiogenesis, and thrombosis?
    Authors: Choudhury A, Freestone B, Patel J, Lip GY
    Chest, 2007-12-01;132(6):1913-9.
    Species: Human
    Sample Types: Plasma
  84. Angiopoietin-2 as a contributing factor of exercise-induced bronchoconstriction in asthmatic patients receiving inhaled corticosteroid therapy.
    Authors: Kanazawa H, Tochino Y, Asai K
    J. Allergy Clin. Immunol., 2007-11-26;121(2):390-5.
    Species: Human
    Sample Types: Sputum
  85. Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia.
    Authors: Lee CY, Tien HF, Hu CY, Chou WC, Lin LI
    Br. J. Cancer, 2007-09-11;97(7):877-82.
    Species: Human
    Sample Types: Plasma
  86. Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
    Authors: Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E
    Br. J. Haematol., 2007-06-01;137(6):560-8.
    Species: Human
    Sample Types: Serum
  87. Serum angiopoietin-2 as a clinical marker for lung cancer.
    Authors: Park JH, Park KJ, Kim YS, Sheen SS, Lee KS, Lee HN, Oh YJ, Hwang SC
    Chest, 2007-05-15;132(1):200-6.
    Species: Human
    Sample Types: Serum
  88. Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells.
    Authors: Vart RJ, Nikitenko LL, Lagos D, Trotter MW, Cannon M, Bourboulia D, Gratrix F, Takeuchi Y, Boshoff C
    Cancer Res., 2007-05-01;67(9):4042-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  89. Roles of angiopoietin-1 and angiopoietin-2 on airway microvascular permeability in asthmatic patients.
    Authors: Kanazawa H, Nomura S, Asai K
    Chest, 2007-04-01;131(4):1035-41.
    Species: Human
    Sample Types: Sputum
  90. Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers.
    Authors: Niedzwiecki S, Stepien T, Kopec K, Kuzdak K, Komorowski J, Krupinski R, Stepien H
    Cytokine, 2007-03-19;36(5):291-5.
    Species: Human
    Sample Types: Serum
  91. Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
    Authors: Caine GJ, Ryan P, Lip GY, Blann AD
    Cancer Lett., 2007-01-19;251(2):296-301.
    Species: Human
    Sample Types: Plasma
  92. Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death.
    Authors: Bhandari V, Choo-Wing R, Lee CG, Zhu Z, Nedrelow JH, Chupp GL, Zhang X, Matthay MA, Ware LB, Homer RJ, Lee PJ, Geick A, de Fougerolles AR, Elias JA
    Nat. Med., 2006-11-05;12(11):1286-93.
    Species: Human
    Sample Types: Plasma
  93. Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures.
    Authors: Fearon U, Mullan R, Markham T, Connolly M, Sullivan S, Poole AR, FitzGerald O, Bresnihan B, Veale DJ
    Arthritis Rheum., 2006-10-01;54(10):3152-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  94. Differential levels of soluble angiopoietin-2 and Tie-2 in patients with haematological malignancies.
    Authors: Quartarone E, Alonci A, Allegra A, Bellomo G, Calabro L, D'Angelo A, Del Fabro V, Grasso A, Cincotta M, Musolino C
    Eur. J. Haematol., 2006-09-15;77(6):480-5.
    Species: Human
    Sample Types: Serum
  95. Circulating levels of total angiopoietin-2 and the soluble Tie-2 receptor in women during ovarian stimulation and early gestation.
    Authors: Molskness TA, Stouffer RL, Burry KA, Gorrill MJ, Lee DM, Patton PE
    Fertil. Steril., 2006-09-14;86(5):1531-3.
    Species: Human
    Sample Types: Serum
  96. Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
    Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
    Cancer Lett., 2006-08-07;248(1):131-6.
    Species: Human
    Sample Types: Plasma
  97. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
    Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
    Br. J. Cancer, 2006-02-27;94(4):524-31.
    Species: Human
    Sample Types: Serum
  98. Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta.
    Authors: Tseng JJ, Hsu SL, Ho ES, Hsieh YT, Wen MC, Chou MM
    Am. J. Obstet. Gynecol., 2006-02-01;194(2):564-71.
    Species: Human
    Sample Types: Tissue Homogenates
  99. Potential role of soluble angiopoietin-2 and Tie-2 in patients with inflammatory bowel disease.
    Authors: Koutroubakis IE, Xidakis C, Karmiris K, Sfiridaki A, Kandidaki E, Kouroumalis EA
    Eur. J. Clin. Invest., 2006-02-01;36(2):127-32.
    Species: Human
    Sample Types: Serum
  100. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005-11-01;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  101. Vitreous levels of angiopoietin 2 and vascular endothelial growth factor in patients with proliferative diabetic retinopathy.
    Authors: Watanabe D, Suzuma K, Suzuma I, Ohashi H, Ojima T, Takagi H
    Am. J. Ophthalmol., 2005-03-01;139(3):476-81.
    Species: Human
    Sample Types: Vitreous Humor
  102. Bi-directional cell contact-dependent regulation of gene expression between endothelial cells and osteoblasts in a three-dimensional spheroidal coculture model.
    Authors: Stahl A, Wenger A, Weber H, Stark GB, Augustin HG, Finkenzeller G
    Biochem. Biophys. Res. Commun., 2004-09-17;322(2):684-92.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human Angiopoietin-2 Quantikine ELISA Kit

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Human Angiopoietin-2 Quantikine ELISA Kit
By Anonymous on 03/24/2022
Sample Tested: Plasma,Serum,Urine

Urine was very low concentration & mostly undetectable at 2-fold. Plasma & Serum showed pretty good linearity up to 20-fold dilution. Almost no matrix effect in plasma at 20-fold dilution.

Human Angiopoietin-2 Quantikine ELISA Kit DANG20
Human Angiopoietin-2 Quantikine ELISA Kit
By Anonymous on 05/16/2020
Sample Tested: Serum
Human Angiopoietin-2 Quantikine ELISA Kit DANG20
Human Angiopoietin-2 Quantikine ELISA Kit
By Anonymous on 09/14/2019
Sample Tested: Recombinant protein
Human Angiopoietin-2 Quantikine ELISA Kit
By Anonymous on 05/24/2019
Sample Tested: Plasma

another excellent ELISA from R and D