Human Angiopoietin-4 Antibody

Catalog #: MAB9641 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9641
MAB9641-SP

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiopoietin-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Angiopoietin-4 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Angiopoietin-1, -2, rhAngiopoietin-like 3, rhAngiopoietin-like 4, rhTie-2, or recombinant mouse Angiopoietin-3 was observed.

Source

Monoclonal Mouse IgG_2B Clone # 156210

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-4
Met1-Ile503
Accession # Q9Y264

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Human Angiopoietin-4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Angiopoietin‑4 Biotinylated Antibody (Catalog # BAF964)

Standard: Recombinant Human Angiopoietin-4 Protein (Catalog # 964-AN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-4

Human Angiopoietin-4 (Ang-4) (1), alternatively named Ang-3 (2), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-4 cDNA encodes a 503 amino acid (aa) precursor protein with a 23 aa signal peptide. It shares 45%, 47% and 54% aa sequence identity with human Ang-1, human Ang-2 and mouse Ang-3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang-1 (97%) and Ang-2 (85%), mouse Ang-3 is believed to be an ortholog of human Ang-4 based on chromosomal localization studies (2). Human Ang-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang-3 is expressed multiple mouse tissues. Human Ang-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse Ang-3 has been reported to be a Tie-2 antagonist. It is likely that mouse Ang-3, like Ang-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

References

Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
Nishimura, M. et al. (1999) FEBS Lett. 448:254.
Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Entrez Gene IDs

51378 (Human)

Alternate Names

AGP4; ANG3; ANG-3; ANG-4; ANG4angiopoietin-3; angiopoietin 4; Angiopoietin-3; Angiopoietin-4; ANGPT4; dJ824F16.2 (angiopoietin 4); MGC138181; MGC138183