Human Angiopoietin-like 3 Antibody

Catalog #: MAB38291 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB38291
MAB38291-SP

All Angiopoietin-like Protein 3/ANGPTL3 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiopoietin-like 3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ANGPTL3 in ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 388620

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin-like 3
Ile19-Glu460
Accession # NP_055310

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human Angiopoietin-like Protein 3/ANGPTL3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody (Catalog # BAF3829)

Standard: Recombinant Human Angiopoietin-like 3 Protein, CF (Catalog # 3829-AN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins (1 - 3). Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do proteolytically separated N- and C-terminal fragments containing the coiled-coil domain and fibrinogen-like domains (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled-coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6 - 8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is upregulated by LXR agonists and downregulated by insulin, leptin, and agonists of TR beta or PPAR beta (11 - 14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (7, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver, also promotes the expansion of hematopoietic stem cells (16). Mature human ANGPTL3 shares 24% - 30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 5, 6, and 7. It shares 77% aa sequence identity with mouse ANGPTL3.

References

Li, C. (2006) Curr. Opin. Lipidol. 17:152.
Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
Kersten, S. (2005) Biochem. Soc. Trans. 33:1059.
Conklin, D. et al. (1999) Genomics 62:477.
Ge, H. et al. (2005) J. Lipid Res. 46:1484.
Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
Koishi, R. et al. (2002) Nat. Genet. 30:151.
Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366.
Koster, A. et al. (2005) Endocrinology 146:4943.
Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
Matsusue, K. et al. (2006) Mol. Cell Endocrinol. 256:23.
Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Alternate Names

AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2