Home / Angiopoietin-like Protein 2/ANGPTL2 / Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

Catalog #: AF2084
1 Citation Datasheet / COA / SDS

Discontinued Product

AF2084 has been discontinued.
View all Angiopoietin-like Protein 2/ANGPTL2 products.
Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot.
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Citations (1)
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Human Angiopoietin-like Protein 2/ANGPTL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Angiopoietin-like 2 (ANGPTL2) in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse ANGPTL2 is observed and less than 1% cross-reactivity with recombinant human (rh) ANGPTL6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-like 2
Gln23-Phe492
Accession # Q9UKU9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 antibody by Western Blot. View Larger

Detection of Human Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot. Western blot shows lysates of human heart tissue, human aorta tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 55-64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 2/ANGPTL2

ANGPTL2, also known as angiopoietin-related protein 2 (ARP2), contains an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, which are structurally conserved in angiopoietins. Unlike angiopoietins which are ligands for TIE2, ANGPTL2 is an orphan ligand that does not bind TIE2.

Entrez Gene IDs
23452 (Human); 26360 (Mouse)
Alternate Names
angiopoietin-like 2; Angiopoietin-like Protein 2; angiopoietin-related protein 2; ANGPTL2; ANGRP2; ARP2HARP; MGC8889

Product Datasheets

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Product Datasheet
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Citation for Human Angiopoietin-like Protein 2/ANGPTL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Knockdown of ANGPTL2 promotes left ventricular systolic dysfunction by upregulation of NOX4 in mice
    Authors: Labbé, P;Martel, C;Shi, YF;Montezano, A;He, Y;Gillis, MA;Higgins, MÈ;Villeneuve, L;Touyz, R;Tardif, JC;Thorin-Trescases, N;Thorin, E;
    Frontiers in physiology
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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