Human Angiopoietin-like Protein 6/ANGPTL6 Antibody

Catalog #: MAB113061 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB113061-100
MAB113061-SP

Human Angiopoietin‑like Protein 6/ANGPTL6 ELISA Standard Curve.

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All Angiopoietin-like Protein 6/ANGPTL6 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiopoietin-like Protein 6/ANGPTL6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Angiopoietin-like protein 6/ANGPTL6 in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 856630

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-expressed recombinant Human Angiopoietin-like Protein 6/ANGPTL6
Arg21-Leu470
Accession # Q8ROZ6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Human Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB11306). This product is intended for assay development on various assay platforms requiring antibody pairs.

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Angiopoietin-like 6 DuoSet ELISA Kit (Catalog # DY7846-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Angiopoietin‑like Protein 6/ANGPTL6 ELISA Standard Curve. Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 protein was serially diluted 2-fold and captured by Mouse Anti-Human Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (MAB11306) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB113061) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 6/ANGPTL6

Angiopoietin-like 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain (aa 51-77,
126-164) and a fibrinogen-like domain (aa 238-456). A conserved Integrin-binding RGD motif is located within the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and promotes insulin sensitivity and energy expenditure in mice fed high fat diets. Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and preeclampsia but are decreased in chronic renal failure. ANGPTL6 is additionally produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.

Entrez Gene IDs

83854 (Human); 70726 (Mouse); 298698 (Rat)

Alternate Names

AGF; AGFAngiopoietin-related protein 5; Angiopoietin like Protein 6; angiopoietin-like 6; Angiopoietin-like Protein 6; Angiopoietin-related growth factor; ANGPTL6; ARP5; ARP5angiopoietin-related protein 6