Home / Angiostatin / Human Angiostatin Kringle 1-3 Biotinylated Antibody

Human Angiostatin Kringle 1-3 Biotinylated Antibody

Catalog #: BAF226
2 Citations Datasheet / COA / SDS
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BAF226
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiostatin Kringle 1-3 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Angiostatin Kringle 1-3 in Western blots. This antibody will also recognize human plasminogen.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Angiostatin Kringle 1-3
Val98-Ser358 (Cys188Ser, Cys316Ser)
Accession # P00747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Angiostatin Kringle 1-3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiostatin

Angiostatin is a 38-45 kDa proteolytic fragment of plasminogen, a 92-100 kDa glycosylated blood zymogen that serves as the precursor for plasmin. Mature human plasminogen is 713 amino acids (aa) in length (aa 98-810). It contains five N-terminal kringle domains (aa 103-561) plus one peptidase S1 domain (aa 581-808). Upon cleavage by tPA between Arg580 and Val581, plasminogen is converted into the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots. Plasminogen is also cleaved by MMPs which generate multiple fragments.  Angiostatin has documented antiangiogenic and antitumor activity, likely mediated by a down‑regulation of Bcl2, and an up‑regulation of thrombospondin-1. Over aa 98-358, human angiostatin shares 81% aa identity with mouse angiostatin.  

Entrez Gene IDs
5340 (Human); 18815 (Mouse)
Alternate Names
Angiostatin; HAE4

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Citations for Human Angiostatin Kringle 1-3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.
    Authors: Hutzen, Brian, Bid, Hemant K, Houghton, Peter J, Pierson, Christop, Powell, Kimerly, Bratasz, Anna, Raffel, Corey, Studebaker, Adam W
    BMC Cancer, 2014-03-19;14(0):206.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Angiostatin production increases in response to decreased nitric oxide in aging rat kidney
    Authors: Minoru Satoh, Kengo Kidokoro, Masahito Ozeki, Hajime Nagasu, Yuko Nishi, Chieko Ihoriya et al.
    Laboratory Investigation

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