Human Annexin A4 Antibody Summary
Met1-Asp319
Accession # P09525
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Annexin A4 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Annexin A4 in Human Kidney. Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and the nuclear envelope of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Annexin A4 by Simple WesternTM. Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Annexin A4 at approximately 41 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin A4
Annexin A4 (ANXA4), also known as Lipocortin IV, placental anticoagulant protein II, and zymogen granule membrane-associated protein of 36 kDa (ZAP36), is a member of the Annexin protein family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Human Annexin A4 shares 92% aa sequence identity with mouse and rat Annexin A4.
Product Datasheets
Citations for Human Annexin A4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Simultaneous membrane binding of Annexin A4 and A5 suppresses 2D lattice formation while maintaining curvature induction
Authors: A Mularski, SL Sønder, ASB Heitmann, J Nylandsted, AC Simonsen
Journal of colloid and interface science, 2021-05-18;600(0):854-864.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair
Authors: TL Boye, K Maeda, W Pezeshkian, SL Sønder, SC Haeger, V Gerke, AC Simonsen, J Nylandsted
Nat Commun, 2017-11-20;8(1):1623.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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