Human Annexin A6 Antibody

Catalog #: MAB5186 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5186
MAB5186-SP

Detection of Human, Mouse, and Rat Annexin A6 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Annexin A6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Annexin A6 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Annexin A1, A2, A3, A4, A8, A9, A10, A11, or A13 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 686921

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Annexin A6
Ala2-Asp673
Accession # P08133

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Annexin A6 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A6 Monoclonal Antibody (Catalog # MAB5186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western

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Detection of Human Annexin A6 by Simple Western^TM. Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687), Exosome Standards (PC‑3) (NBP2-49856) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Annexin A6 at approximately 72 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Annexin A6 Monoclonal Antibody (Catalog # MAB5186). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Immunohistochemistry

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Annexin A6 in Human Placenta. Annexin A6 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Annexin A6 Monoclonal Antibody (Catalog # MAB5186) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to membranes of syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Annexin A6

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60-70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5‑7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

References

Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
Crompton, M.R. et al. (1988) EMBO J. 7:21.
Smith, P.D. et al. (1994) Proc. Natl. Acad. Sci. 91:2713.
Grewal, T. et al. (2006) Bioessays 28:1211.
Thorin, B. et al. (1995) Biochem. Biophys. Res. Commun. 209:1039.
Takagi, H. et al. (2002) J. Cell Sci. 115:3309.
Tressler, R.J. et al. (1994) Exp. Cell Res. 215:395.
Ishitsuka, R. et al. (1998) J. Biol. Chem. 273:9935.

Entrez Gene IDs

309 (Human); 11749 (Mouse); 79125 (Rat)

Alternate Names

67 kDa calelectrin; Annexin A6; annexin VI (p68); Annexin VI; Annexin-6; ANX6; ANXA6; calcium-binding protein p68; Calelectrin; calphobindin II; calphobindin-II; CBP68; chromobindin-20; CPB-II ; CPB-II; Lipocortin VI; p68; p70; Protein III