Human AP-2 epsilon Antibody

Catalog #: AF5060
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5060
AF5060-SP

Detection of Human AP‑2 epsilon by Western Blot.

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All AP-2 epsilon Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AP-2 epsilon Antibody Summary

Species Reactivity

Human

Specificity

Detects human AP-2 epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) AP‑2 alpha, rhAP‑2 beta, rhAP‑2δ, and rhAP‑2 gamma is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human AP-2 epsilon
Ala123-Val217
Accession # Q6VUC0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human AP‑2 epsilon by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP-2e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP-2e at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AP-2 epsilon

AP-2 epsilon (Activating protein 2 epsilon) is a 48 kDa member of the AP-2 transcription factor family. It is found in embryonic olfactory bulb mitral cells and adult keratinocytes and chondrocytes. In the nucleus, it presumably forms homodimers and heterodimers with other AP‑2 family members. Human AP‑2 epsilon is 442 amino acids in length. It contains a Gln/Pro-rich transactivation domain (aa 22‑122), a DNA binding domain (aa 216‑286) and an AP‑2, helix‑span‑helix dimerization region (aa 287‑417). There is one potential alternate start site at Met09. Over aa 123‑217, human AP‑2 epsilon shares 86% aa identity with mouse AP‑2 epsilon.

Long Name

Activating Enhancer-binding Protein epsilon

Entrez Gene IDs

339488 (Human)

Alternate Names

Activating enhancer-binding protein 2-epsilon; adaptor-related protein complex 2, epsilon subunit; AP2 epsilon; AP-2 epsilon; AP2E; AP2-epsilon; MGC49007; TFAP2E; transcription factor AP-2 epsilon (activating enhancer binding protein 2epsilon); transcription factor AP-2-epsilon

Product Datasheets

Product Datasheet

COA

SDS

Citations for Human AP-2 epsilon Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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