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Human APAF-1 Antibody

Catalog #: MAB868
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB868
MAB868-SP
Detection of Human APAF‑1 by Western Blot.
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Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APAF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human APAF-1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 94408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human APAF-1
Leu10-Asp214
Accession # AAC51678
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human APAF‑1 antibody by Western Blot. View Larger

Detection of Human APAF‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105cells (lane 1) and 7 x 104cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF-1 Monoclonal Antibody (Catalog # MAB868) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for APAF-1 was detected at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APAF-1

Apoptosis protease-activating factor 1 (APAF‑1) is a ubiquitous 135 kDa protein component of the cytoplasmic apoptosome complex. APAF‑1 plays an important role in the mitochondrial apoptotic pathway by triggering the Cytochrome c-dependent activation of Caspase-9. APAF‑1 participates in tissue morphogenesis during development, and loss of APAF‑1 expression is associated with tumor progression in a variety of cancers. Some alternate splice forms of APAF‑1 are deficient in their ability to promote apoptosis.

Long Name
Apoptotic Peptidase Activating Factor 1
Entrez Gene IDs
317 (Human)
Alternate Names
APAF1; APAF-1; APAF-1apoptotic protease activating factor 1; apoptotic peptidase activating factor 1; apoptotic peptidase activating factor; apoptotic protease activating factor; apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413

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Citations for Human APAF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors
    Authors: AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack
    Int J Mol Sci, 2020-11-05;21(21):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Delayed apoptosis allows islet ?-cells to implement an autophagic mechanism to promote cell survival
    Authors: HL Hayes, BS Peterson, JM Haldeman, CB Newgard, HE Hohmeier, SB Stephens
    PLoS ONE, 2017-02-17;12(2):e0172567.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters.
    Authors: Mungamuri S, Wang S, Manfredi J, Gu W, Aaronson S
    Oncogene, 2014-07-14;34(19):2461-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis.
    Authors: Kracikova, M, Akiri, G, George, A, Sachidanandam, R, Aaronson, S A
    Cell Death Differ, 2013-01-11;20(4):576-88.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Alteration of the nuclear pore complex in Ca(2+)-mediated cell death.
    Authors: Bano D, Dinsdale D, Cabrera-Socorro A, Maida S, Lambacher N, McColl B, Ferrando-May E, Hengartner MO, Nicotera P
    Cell Death Differ., 2010-01-01;17(1):119-33.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  6. Intracellular K(+) inhibits apoptosis by suppressing the Apaf-1 apoptosome formation and subsequent downstream pathways but not cytochrome c release.
    Authors: Karki P, Seong C, Kim JE, Hur K, Shin SY, Lee JS, Cho B, Park IS
    Cell Death Differ., 2007-09-21;14(12):2068-75.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells.
    Authors: Sato T, Machida T, Takahashi S, Iyama S, Sato Y, Kuribayashi K, Takada K, Oku T, Kawano Y, Okamoto T, Takimoto R, Matsunaga T, Takayama T, Takahashi M, Kato J, Niitsu Y
    J. Immunol., 2004-07-01;173(1):285-96.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. The role of mitochondria, cytochrome c and caspase-9 in embryonic lens fibre cell denucleation.
    Authors: Sanders EJ, Parker E
    J. Anat., 2002-08-01;201(2):121-35.
    Species: Chicken
    Sample Types: Cell Lysates
    Applications: Western Blot

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