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Human APJ APC-conjugated Antibody

Catalog #: FAB8561A
3 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8561A-025
Detection of APJ in U‑118‑MG Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APJ APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human APJ in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 72133R
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human APJ
Met1-Asp380
Accession # P35414
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of APJ antibody in U-118-MG Human Cell Line antibody by Flow Cytometry. View Larger

Detection of APJ in U‑118‑MG Human Cell Line by Flow Cytometry. U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human APJ APC-conjugated Monoclonal Antibody (Catalog # FAB8561A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: APJ

APJ is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, apelin. APJ can also function as a co-receptor for HIV.

Long Name
Apelin Receptor/G-protein Coupled Receptor APJ
Entrez Gene IDs
187 (Human); 23796 (Mouse); 83518 (Rat)
Alternate Names
AGTRL1; AGTRL1HG11; angiotensin II receptor-like 1; Angiotensin receptor-like 1; apelin receptor; APJ (apelin) receptor; APJ receptor; APJ; APJFLJ96609; APJR; APLNR; FLJ90771; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11 orphan receptor; HG11; MGC45246

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Citations for Human APJ APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lateral plate mesoderm cell-based organoid system for NK cell regeneration from human pluripotent stem cells
    Authors: D Huang, J Li, F Hu, C Xia, Q Weng, T Wang, H Peng, B Wu, H Wu, J Xiong, Y Lin, Y Wang, Q Zhang, X Liu, L Liu, X Zheng, Y Geng, X Du, X Zhu, L Wang, J Hao, J Wang
    Cell Discovery, 2022-11-08;8(1):121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Temporal perturbation of histone deacetylase activity reveals a requirement for HDAC1-3 in mesendoderm cell differentiation
    Authors: E Sinniah, Z Wu, S Shen, M Naval-Sanc, X Chen, J Lim, A Helfer, A Iyer, J Tng, AJ Lucke, RC Reid, MA Redd, CM Nefzger, DP Fairlie, NJ Palpant
    Cell Reports, 2022-05-17;39(7):110818.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice
    Authors: CH Gil, D Chakrabort, CP Vieira, N Prasain, SL Calzi, SD Fortmann, P Hu, K Banno, M Jamal, C Huang, MS Sielski, Y Lin, X Huang, MD Dupont, JL Floyd, R Prasad, ALF Longhini, TJ McGill, HM Chung, MP Murphy, DN Kotton, ME Boulton, MC Yoder, MB Grant
    Science Advances, 2022-03-04;8(9):eabm5559.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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