Human Apolipoprotein H/ApoH Antibody

Catalog #: AF5087 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5087
AF5087-SP

Detection of Human Apolipoprotein H/ApoH by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein H/ApoH Antibody Summary

Species Reactivity

Human

Specificity

Detects human Apolipoprotein H/ApoH in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Apolipoprotein H/ApoH
Gly20-Cys345
Accession # P02749

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human ApoH (5 ng) was included. A specific band for Apolipoprotein H/ ApoH was detected at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry

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Apolipoprotein H/ApoH in Mouse Pancreas. Apolipoprotein H/ApoH was detected in perfusion fixed frozen sections of adult mouse pancreas using Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western

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Detection of Human Apolipoprotein H/ApoH by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 69 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein H/ApoH

ApoH (Apolipoprotein H; also beta 2-Glycoprotein 1/ beta 2-GPI) is a 50 kDa, secreted, variably glycosylated, monomeric member of the complement control superfamily of molecules. It is produced by hepatocytes and is bound to HDL particles. Circulating ApoH blocks the intrinsic clotting pathway, and binds to exposed phospholipids on apoptotic cells. Mature human ApoH is 326 amino acids (aa) in length (aa 20‑345). It contains four Sushi repeats (aa 21‑260) and one C-terminal kringle domain (aa 261‑345) that binds heparin and phospholipids. At least two ApoH isoforms exist, one showing a 31 aa substitution for aa 140‑345, while a second represents a bioactive plasmin cleavage product that removes the C-terminal nine amino acids. Mature human ApoH shares 76% aa identity with mouse ApoH.

Entrez Gene IDs

350 (Human); 11818 (Mouse); 287774 (Rat)

Alternate Names

Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; APOH; apo-H; apolipoprotein H (beta-2-glycoprotein I); Apolipoprotein H; B2G1; B2GP1; B2GPI; Beta; beta(2)GPI; beta-2-glycoprotein 1; Beta-2-glycoprotein I; BG