Human Apolipoprotein M/ApoM Antibody

Catalog #: AF4550 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4550
AF4550-SP

Detection of Human Apolipoprotein M/ApoM by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein M/ApoM Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human ApoM in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ApoM
Cys23-Asn188
Accession # O95445

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Apolipoprotein M/ApoM by Western Blot. Western blot shows lysates of human plasma and liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein M/ApoM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4550) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein M/ApoM at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein M/ApoM

Apolipoprotein M (ApoM; also known as Protein G3a) is a 25 kDa, secreted member of the lipocalin family of proteins. It is produced by hepatocytes and renal tubular epithelium, and represents a minor component of HDL particles. Human ApoM is 188 amino acids (aa) in length. It contains an uncleaved, hydrophobic N-terminal segment and one utilized N-linked glycosylation site (mouse ApoM shows no glycosylation). At least two potential isoforms exist. One shows an alternate start site at Met73, while a second shows the same start site plus a 21 aa substitution for aa 181‑188. Human ApoM shares 91% and 80% aa identity with pig and mouse ApoM, respectively.

Entrez Gene IDs

55937 (Human); 55938 (Mouse); 55939 (Rat)

Alternate Names

Apolipoprotein M; APOM; apo-M; G3A; HSPC336; MGC22400; NG20; NG20-like protein; Protein G3a