Home / ARX / Human ARX Antibody

Human ARX Antibody

Catalog #: AF7068
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7068
AF7068-SP
Detection of Human ARX by Western Blot.
3 Images
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ARX Antibody Summary

Species Reactivity
Human
Specificity
Detects human ARX in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ARX
Ser2-Ala100
Accession # Q96QS3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ARX antibody by Western Blot. View Larger

Detection of Human ARX by Western Blot. Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ARX antibody in U-87 MG Human Cell Line by Immunocytochemistry (ICC). View Larger

ARX in U‑87 MG Human Cell Line. ARX was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ARX antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

ARX in Human Pancreas. ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARX

ARX (Aristaless-Related homeoboX protein) is a 56-62 kDa member of the aristaless subfamily, paired homeobox family of proteins. In the nervous system, it is expressed in embryonic telencephalon plus adult cortical GABAergic neurons and differentiating neuronal stem cells; in the pancreas, it promotes the differentiation of alpha -cells at the expense of beta -cells. Human ARX is a 562 amino acid (aa) transcriptional repressor. It contains an octapeptide motif that binds Groucho proteins (aa 27‑34), an NLS (aa 82-89), one Ala-rich region (aa 100-155), an acidic/Glu-rich region (aa 224-253), a DNA-binding homeodomain with an embedded NLS (aa 328‑387), one Pro-rich segment that binds CtBP1 (aa 395-459) and a C-terminal Aristaless domain (aa 530-543). There are mutations that show poly-Ala expansions in the N-terminus (aa 115-155), often associated with developmental abnormalities. Over aa 1-100, human ARX shares 97% aa identity with mouse ARX.

Long Name
Aristless-related Homeobox Protein
Entrez Gene IDs
170302 (Human); 11878 (Mouse); 317268 (Rat)
Alternate Names
aristaless related homeobox; Aristaless-related homeobox; aristaless-related homeobox, X-linked; ARX; cancer/testis antigen 121; CT121; EIEE1; homeobox protein ARX; ISSX; ISSXmental retardation, X-linked 36; mental retardation, X-linked 29; mental retardation, X-linked 32; mental retardation, X-linked 33; mental retardation, X-linked 38; mental retardation, X-linked 43; mental retardation, X-linked 54; mental retardation, X-linked 76; mental retardation, X-linked 87; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRX76; MRX87; MRXS1; PRTS

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human ARX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Metastatic insulinoma: exploration from clinicopathological signatures and genetic characteristics
    Authors: Jingcheng Zhang, Rui Jiang, Xiafei Hong, Huanwen Wu, Xianlin Han, Wenming Wu
    Frontiers in Oncology
  2. Highly Proliferative alpha-Cell-Related Islet Endocrine Cells in Human Pancreata.
    Authors: Lam CJ, Cox AR, Jacobson DR et al.
    Diabetes
  3. Distinct hyperactive RAS/MAPK alleles converge on common GABAergic interneuron core programs
    Authors: Sara J. Knowles, April M. Stafford, Tariq Zaman, Kartik Angara, Michael R. Williams, Jason M. Newbern et al.
    Development
  4. DNA methylation reveals distinct cells of origin for pancreatic neuroendocrine carcinomas and pancreatic neuroendocrine tumors
    Authors: Tincy Simon, Pamela Riemer, Armin Jarosch, Katharina Detjen, Annunziata Di Domenico, Felix Bormann et al.
    Genome Medicine
  5. Heterogenous impairment of alpha cell function in type 2 diabetes is linked to cell maturation state
    Authors: Xiao-Qing Dai, Joan Camunas-Soler, Linford J.B. Briant, Theodore dos Santos, Aliya F. Spigelman, Emily M. Walker et al.
    Cell Metabolism
  6. Integrated Analysis of the Pancreas and Islets Reveals Unexpected Findings in Human Male With Type 1 Diabetes
    Authors: Rachana Haliyur, John T Walker, May Sanyoura, Conrad V Reihsmann, Shristi Shrestha, Radhika Aramandla et al.
    Journal of the Endocrine Society
  7. Epigenetic landscape of pancreatic neuroendocrine tumours reveals distinct cells of origin and means of tumour progression
    Authors: Annunziata Di Domenico, Christodoulos P. Pipinikas, Renaud S. Maire, Konstantin Bräutigam, Cedric Simillion, Matthias S. Dettmer et al.
    Communications Biology
  8. Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein
    Authors: X Shi, W Lin, X Gao, W Xie, JA Golden, T Tao
    Biosci. Rep., 2020-07-31;40(7):.
    Species: Human
    Sample Types: Cell culture supernate
  9. Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)
    Authors: N Moya, A Shahryari, I Burtscher, J Beckenbaue, M Bakhti, H Lickert
    Stem Cell Res, 2020-06-09;46(0):101874.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced
    Authors: P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren
    J Pathol Clin Res, 2019-09-07;5(4):248-255.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human ARX Antibody

There are currently no reviews for this product. Be the first to review Human ARX Antibody and earn rewards!

Have you used Human ARX Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review