Home / Arylsulfatase B/ARSB / Human Arylsulfatase B/ARSB Antibody

Human Arylsulfatase B/ARSB Antibody

Catalog #: MAB4415
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4415
MAB4415-SP
Detection of Human Arylsulfatase B/ARSB by Western Blot.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Arylsulfatase B/ARSB Antibody Summary

Species Reactivity
Human
Specificity
Detects human Arylsulfatase B/ARSB in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ARSA, rhARSJ, or recombinant mouse ARSG is observed.
Source
Monoclonal Mouse IgG1 Clone # 708401
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Arylsulfatase B/ARSB
Ser37-Met533
Accession # P15848
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Arylsulfatase B/ARSB antibody by Western Blot. View Larger

Detection of Human Arylsulfatase B/ARSB by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Arylsulfatase B/ARSB at approximately 47 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Arylsulfatase B/ARSB antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Arylsulfatase B/ARSB in HepG2 Human Cell Line. Arylsulfatase B/ARSB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arylsulfatase B/ARSB

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3).

References
  1. Peters, C. et al. (1990) J. Biol. Chem. 265:3374.
  2. O’Brien, J.S. et al. (1974) Biochem. Biophys. Res. Commun. 60:1170.
  3. Wicker, G. et al. (1991) J. Biol. Chem. 266:21386.
Entrez Gene IDs
411 (Human); 11881 (Mouse); 25227 (Rat)
Alternate Names
ARSB; Arylsulfatase B; ASB; EC 3.1.6.12; G4S; MPS6; N-acetylgalactosamine-4-sulfatase

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Citations for Human Arylsulfatase B/ARSB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Correction: Nuclear Legumain Activity in Colorectal Cancer
    Authors: Mads H. Haugen, Harald T. Johansen, Solveig J. Pettersen, Rigmor Solberg, Klaudia Brix, Kjersti Flatmark et al.
    PLoS ONE
  2. Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions
    Authors: NN Lunde, MH Haugen, KB Bodin Lars, I Damgaard, SJ Pettersen, R Kasem, W Rut, M Drag, M Poreba, HT Johansen, R Solberg
    Biochimie, 2017-05-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Simvastatin inhibits glucose metabolism and legumain activity in human myotubes.
    Authors: Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H
    PLoS ONE, 2014-01-08;9(1):e85721.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Nuclear Legumain Activity in Colorectal Cancer
    Authors: Mads H. Haugen, Harald T. Johansen, Solveig J. Pettersen, Rigmor Solberg, Klaudia Brix, Kjersti Flatmark et al.
    PLoS ONE

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